1,4-bis(vinyloxy)butane

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1973-12-18 to 1975-03-06

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Scientifically acceptable, method eqivalent or similar to guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1975

Report date:

1975

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Version / remarks:

method applied according to BASF-internal standard

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,4-bis(vinyloxy)butane

EC Number:

223-437-0

EC Name:

1,4-bis(vinyloxy)butane

Cas Number:

3891-33-6

Molecular formula:

C8H14O2

IUPAC Name:

1,4-bis(ethenyloxy)butane

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Gaukler, 6050 Offenbach/Main, Germany
- Age at study initiation: no data
- Weight at study initiation: 2.7 - 3.0 kg
- Housing: no data
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: no data

ENVIRONMENTAL CONDITIONS
- Temperature (°C): no data
- Humidity (%): no data
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): no data

IN-LIFE DATES: From: 1973-12-18 To: 1973-12-27

Test system

Type of coverage:

occlusive

Preparation of test site:

clipped

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated skin of the same animal

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied: ca. 1 mL

Duration of treatment / exposure:

The test substance was applied for 1 min, 5 min, 15 min and 20 h

Observation period:

8 days

Number of animals:

2

Details on study design:

TEST SITE
- Area of exposure: 2.5 x 2.5 cm
- Type of wrap if used:
Application to ear: a cotton ball soaked with test item was fixed to the ear
Application to back: a small piece of cloth was fixed to the back with the help of a gauze patch

REMOVAL OF TEST SUBSTANCE
- Washing: After the short-term applications the test substance was washed off with Lutrol; after the 20-hour application the test substance was not washed off.
- Time after start of exposure: 1 min, 5 min and 15 min

SCORING SYSTEM: observed were the parameters erythema, edema, scaling, and necrosis
Grading system:
"reizlos" Ø: considered as equivalent to Grade 0 (no irritating effect) according to OECD Guideline 404
"fraglich" (+): considered as equivalent to Grade 1 (very slight erythema (barely perceptible)) according to OECD Guideline 404
"leicht" +: considered as equivalent to Grade 2 (well-defined effect) according to OECD Guideline 404
"stark" ++: considered as equivalent to Grade 3 (moderate (to severe)) effect according to OECD Guideline 404
"sehr stark" +++: considered as equivalent to Grade 4 (severe effect) according to OECD Guideline 404

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 24 h

Score:

2

Max. score:

4

Reversibility:

other: incipient scaling after 8 days

Remarks on result:

other: 20-hour application to back

Results 2

Irritation parameter:

erythema score

Basis:

animal #1

Time point:

other: 24 h

Score:

2

Max. score:

4

Reversibility:

other: incipient scaling after 8 days

Remarks on result:

other: 20-hour application to back

Results 3

Irritation parameter:

erythema score

Basis:

animal #2

Time point:

other: 24 h

Score:

2

Max. score:

4

Reversibility:

other: incipient scaling after 8 days

Remarks on result:

other: 20-hour application to back

Results 4

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 24 h

Score:

2

Max. score:

4

Reversibility:

other: incipient scaling after 8 days

Remarks on result:

other: 20-hour application to back

Results 5

Irritation parameter:

edema score

Basis:

animal #1

Time point:

other: 24 h

Score:

2

Max. score:

4

Reversibility:

other: incipient scaling after 8 days

Remarks on result:

other: 20-hour application to back

Results 6

Irritation parameter:

edema score

Basis:

animal #2

Time point:

other: 24 h

Score:

2

Max. score:

4

Reversibility:

other: incipient scaling after 8 days

Remarks on result:

other: 20-hour application to back

Irritant / corrosive response data:

Application to ear for 20 hours resulted in Grade 3 erythema and edema after 24 hours. Erythema and edema not reported on day 8, however eschar was observed.

Any other information on results incl. tables

Table of results (20 h application of test item) based on grading system as applied in the study report

   

	time	animal 1	animal 2
erythema	24 h	+	+
	8 d	Ø	Ø
edema	24 h	+	+
	8 d	Ø	Ø
scaling	24 h	Ø	Ø
	8 d	+	+

 

Table of results (20 h application of test item) translated into the grading system as applied in OECD Guideline 404

  

	time	animal 1	animal 2
erythema	24 h	2	2
	8 d	0	0
edema	24 h	2	2
	8 d	0	0
scaling	24 h	0	0
	8 d	2	2

 

Applicant's summary and conclusion

Interpretation of results:

slightly irritating

Remarks:

Migrated information