Reaction products of bis(4-methylpentan-2-yl)dithiophosphoric acid with phosphorus oxide, propylene oxide and amines, C12-14-alkyl (branched)

Stability in organic solvents and identity of relevant degradation products

Link to relevant study record(s)

Reference

Endpoint:

stability in organic solvents and identity of relevant degradation products

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: No specific guideline for this endpoint are available. The study was performed under GLP conditions by an analytical method (GC) which was validated with respect to linearity of instrument response and recovery of test material.

Qualifier:

no guideline available

Principles of method if other than guideline:

The stability of test material in Arachis Oil BP (vehicle used in a Reproduction /Developmental Toxicity Screening ) was determined by GC (gas chromatography). The analytical method was evaluated for linearity of GC detector response and % recovery from column.

Chromatograms of the test substance in Arachis Oil BP were compared immediately after preparation and then again after storage at ambient conditions. Chromatograms were compared both qualitatively (by retention times of eluted peaks) and quantitatively on the basis of peak areas. Results of the analysis are used as the basis for determining if the test material is stable in the vehicle for the duration of study.

GLP compliance:

yes

Test substance stable:

yes

Transformation products:

not specified

GC results show the formulations to be stable in Arachis Oil for at least four hours. Table 3 gives a comparison of the iniial concentration and the concentration of test material after 4 hours

Table 3: Stability of test material in Arachis Oil BP after 4 hours

Nominal (mg/mL)	Initial Concentration (mg/mL)	Concentration After 4 Hours
		mg/mL	as % of initial concentraton)
3.75	3.85	4.06	105
37.5	35.9	36.1	104
125	121	127	107

Conclusions:

Results show the formulations to be stable for at least four hours. Formulations were therefore prepared daily and stored at approximately +4ºC in the dark, under nitrogen, over silica gel until use within 4 hours.

Executive summary:

The stability of test material in Arachis Oil BP (the vehicle used in Reproduction/Developmental Toxicity Screening ) was determined by GC (gas chromatography). A comparison of the peak area responses showed solutions of the test material in Arachis Oil BP were stable for at least four hours. Formulations were therefore prepared daily and stored at approximately +4 ºC in the dark, under nitrogen, over silica gel until use within 4 hours.

Description of key information

Solutions of the test material in Arachis Oil BP were stable for at least four hours (analysis associated with OECD 422)

Additional information

The stability of test material in Arachis Oil BP (the vehicle used in Reproduction/Developmental Toxicity Screening ) was determined by GC (gas chromatography). A comparison of the peak area responses showed solutions of the test material in Arachis Oil BP were stable for at least four hours. Formulations were therefore prepared daily and stored at approximately +4ºC in the dark, under nitrogen, over silica gel until use within 4 hours.