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EC number: 931-384-6 | CAS number: -
Disintegrations per Minute, Disintegrations per Minute/Node and Stimulation Index
Concentration (% w/w) in
Acetone/olive oil 4:1
Dpm = disintegrations per minutes
a = total number of lymph nodes per animals is 2
b = Stimulation Index of 3.0 or greater indicates a positive result
N/A = Not Applicable
Individual clinical Observations and Mortality Data
Conc (%v/v) in acetone/olive oil 4:1
0 = No signs of systemic toxicity
Individual Bodyweights and Bodyweight Changes
Bodyweight Change (g)
Introduction.A study was performed to assess the skin sensitization potential of the test material in the CBA/Ca strain mouse following topical application to the dorsal surface of the ear. The method was designed to meet the requirements OECD 429 and Method B42.
Methods.Following a preliminary screening test in which no clinical signs of toxicity were noted at a concentration of 50% w/w, this concentration was selected as the highest dose investigated in the main test of the Local Lymph Node Assay. Three groups, each of four animals, were treated with 50 μl (25 μl per ear) of the test material as a solution in acetone/olive oil 4:1 at concentrations of 50%, 25% or 10% w/w. A further group of four animals was treated with acetone/olive oil 4:1 alone.
Results.The Stimulation Index expressed as the mean radioactive incorporation for each treatment group divided by the mean radioactive incorporation of the vehicle control group are as follows:
acetone/olive oil 4:1
According to the calculation the concentration of test material expected to cause a 3 fold increase in 3HTdR incorporation (EC3 value) was found to be 9.39%.
Conclusion.The test material was considered to be a sensitiser under the conditions of the test.
EC3 value was determined to be 9.39%. Per the CLP guidance, substances are to be classified as skin sensitization 1A when the EC3 value is less than 2% and are to be classified as skin sensitization 1B when the EC3 value is greater than 2%.
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