Ethylenediamine

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Qualifier:

no guideline followed

Principles of method if other than guideline:

Respiration inhibition test on nitrifying bacteria

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Details on test solutions:

A stock solution was prepared in demineralized water and neutralised to a pH between 7.0 and 7.3.

Test organisms (species):

other: nitrifying bacteria

Details on inoculum:

- Laboratory culture: In house continuous culture, 1.4 g dissolved solids/L

Test type:

static

Water media type:

freshwater

Total exposure duration:

2 h

Test temperature:

19 - 21°C

pH:

8.4 - 8.8

Nominal and measured concentrations:

0.5, 1.0, 5.6, 10 and 200 mg/L

Details on test conditions:

Respiration activity was measured in an open respirometer filled with 100 mL bacteria suspension. Oxygen concentration was measured with an oxygen electrode.

Reference substance (positive control):

no

Duration:

2 h

Dose descriptor:

EC10

Effect conc.:

0.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Duration:

2 h

Dose descriptor:

EC50

Effect conc.:

3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: 0.6 - 5.7 mg/L

Validity criteria fulfilled:

not applicable

Conclusions:

The 2 h NOEC for inhibition of respiration rate of nitrifying bacteria was 0.5 mg/L and the 2 h EC50 was 3.2 mg/L.

Executive summary:

A study was conducted to determine the toxicity of the test substance on the respiration of nitrifying bacteria. No guideline was followed. The respiration activity of bacteria exposed to the test substance during two hours was compared to a control and the inhibition was calculated. From the relation between the concentration and the inhibition percentages, the EC50 was determined. The respiration activity was not significantly inhibited by the concentration of 0.5 mg/L (EC 10). Almost complete inhibition was obtained at 10 mg/L. Under the study conditions, the EC50 was determined to be 3.2 mg/L, with 95% confidence limits of 0.6 and 5.7 mg/L (Balk, 1989).

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

according to guideline

Guideline:

other: EC protocol as published O.J. 30 May 1988

Deviations:

yes

Remarks:

choice of control substance

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Details on test solutions:

Stock solution of of test compound was titrated to a neutral pH.

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

Activated sludge from RZWI Nieuwgraaf in Duiven.

Test type:

static

Water media type:

freshwater

Total exposure duration:

30 min

Test temperature:

20°C

pH:

7.1 -7.8

Nominal and measured concentrations:

0, 20, 60, 180, 540 and 620 mg/L

Details on test conditions:

Activated sludge concentration 0.46 dry/weight /L.
Volume of test vessel: 10 mL

Reference substance (positive control):

yes

Remarks:

1,4,5 - trichlorophenol

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

20 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

1 600 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Validity criteria fulfilled:

yes

Conclusions:

30 min EC20 for inhibition of activated sludge is 20 mg/L and the EC50 is 1600 mg/L.

Executive summary:

A study was conducted to determine the toxicity of the test substance to activated sludge from predominantly domestic sewage according to the EC protocol as published in the Official Journal of 30  May 1988, in compliance with GLP. The potential effect on the inhibition of respiration rate was assessed. The nominal test concentrations were 0, 20, 60, 180, 540 and 620 mg/L during a 30 min exposure period. Under the study conditions, the 30 min EC20 and EC50 were determined to be 20 and 1600 mg/L, respectively (van Ginkel, 1989).

Reference 3

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

other: ISO/TC 147/SC 5/WG 1

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Details on test solutions:

The test substance was dissolved in growth medium and titrated to neutral pH using 1M sulfuric acid.

Test organisms (species):

Pseudomonas putida

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

17 h

Test temperature:

28°C

Nominal and measured concentrations:

0, 3.1, 6.3, 12.5, 25.0, 50.0 and 100 mg/L nominal concentrations

Duration:

17 h

Dose descriptor:

EC10

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Duration:

17 h

Dose descriptor:

EC50

Effect conc.:

29 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Validity criteria fulfilled:

yes

Conclusions:

In a 17 h inhibition test to Pseudomonas putida the EC10 was 10 mg/L and the EC50 was 29 mg/L.

Executive summary:

A study was conducted to determine the toxicity of the test substance to Pseudomonas putida according to ISO/TC 147/SC 5/WG 1, in compliance with GLP. The growth inhibition of P. putida was assessed over an exposure period of 17 h. In the definitive test, the microorganisms were exposed to nominal concentrations of 0, 3.1, 6.3, 12.5, 25, 50 and 100 mg/L. Under the study conditions, the 17 h EC10 and EC50 were determined to be 10 and 29 mg/L, respectively (van Ginkel, 1989).

Key value for chemical safety assessment

EC50 for microorganisms:

3.2 mg/L

EC10 or NOEC for microorganisms:

0.5 mg/L

Additional information

A study was conducted to determine the effect of the test substance on the respiration of nitrifying bacteria. No guideline was followed. The respiration activity of bacteria exposed to the test substance during 2 h was compared to a control and inhibition was calculated. From the relation between the concentration and the inhibition percentages, the EC50 was determined. The respiration activity was not significantly inhibited at a concentration of 0.5 mg/L (EC10). Almost complete inhibition was obtained at 10 mg/L. Under the study conditions, the EC50 was determined to be 3.2 mg/L, with 95% confidence limits of 0.6 and 5.7 mg/L (Balk, 1989).

A study was conducted to determine the toxicity of the test substance to activated sludge from predominantly domestic sewage according to the EC protocol as published in the Official Journal of 30  May 1988, in compliance with GLP. The potential effect on the inhibition of respiration rate was assessed. The nominal test concentrations were 0, 20, 60, 180, 540 and 620 mg/L during a 30 min exposure period. Under the study conditions, the 30 min EC20 and EC50 were determined to be 20 and 1600 mg/L, respectively (van Ginkel, 1989).

A study was conducted to determine the toxicity of the test substance to Pseudomonas putida according to ISO/TC 147/SC 5/WG 1, in compliance with GLP. The growth inhibition of P. putida was assessed over an exposure period of 17 h. In the definitive test, the microorganisms were exposed to nominal concentrations of 0, 3.1, 6.3, 12.5, 25, 50 and 100 mg/L. Under the study conditions, the 17 h EC10 and EC50 were determined to be 10 and 29 mg/L, respectively (van Ginkel, 1989).