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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in mammalian cells
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1996

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The mutagenicity of lead chloride was examined in Chinese hamster ovary AS52 cells at concentrations from 0.1 - 1.0 uM.
GLP compliance:
not specified
Type of assay:
mammalian cell gene mutation assay

Test material

Reference
Name:
Unnamed
Type:
Constituent

Results and discussion

Test results
Species / strain:
Chinese hamster Ovary (CHO)
Metabolic activation:
not specified
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
cytotoxicity
Vehicle controls validity:
valid
Untreated negative controls validity:
not examined
Positive controls validity:
not examined
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

Lead chloride had little effect on cell viability up to concentrations of 0.5 uM. Lead chloride induced a dose-dependent increase in the isolation of mutants resistant to 6-thioguanine (TG) compared to controls.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
positive
Executive summary:

The mutagenicity of lead chloride was examined in Chinese hamster ovary AS52 cells at concentrations from 0.1 - 1.0 uM. Lead chloride had little effect on cell viability up to concentrations of 0.5 uM. Lead chloride induced a dose-dependent increase in the isolation of mutants resistant to 6-thioguanine (TG) compared to controls.