Registration Dossier
Registration Dossier
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EC number: 267-007-0 | CAS number: 67762-26-9 This substance is identified by SDA Substance Name: C14-C18 and C16-C18 unsaturated alkyl carboxylic acid methyl ester and SDA Reporting Number: 04-010-00.
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- November 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study not in GLP, purity of the sample has not been assessed, but the study is conducting following official guideline and scientifically valid to assessing the end point
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Details on sampling:
- - Sample storage conditions before analysis:internal laboratory storage
- Vehicle:
- no
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Source : culture collection of Algae and protozoa-Cumbria U.D. CCAP n° 27814 cultured in the internal lab - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 25°C +/-2°C
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 73 729 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Conclusions:
- After an incubation of 72 hours , there is a 50% growth inibithion of Selenastrum capricornum for 73729 mg/L of substance
- Executive summary:
Fatty acid, rape oil, methyl ester has been tested for toxicity to aquatic algae according to OECD 201, on selenastrum capricornutum. Behaviour of algae is similar to that of bacteria, and Water Soluble Fractions has been prepared starting from WAF 10%.
Reference
| Concentration | Optical density after 72h | Mean | % Inhibition | ||
| A | B | C | |||
| Control | 1.02 | 1.03 | 1.01 | 1.02 | - |
| WSF 0.001% | 1.02 | 1.02 | 1.02 | 1.02 | 0.97 |
| WSF 0.01% | 1.02 | 1.01 | 1.00 | 1.01 | 1.76 |
| WSF 0.1% | 1.01 | 1.00 | 0.99 | 1.00 | 2.01 |
| WSF 1% | 0.85 | 0.85 | 0.84 | 0.85 | 12.68 |
| WSF 10% | 0.31 | 0.3 | 0.32 | 0.31 | 66.54 |
Description of key information
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 73 729 mg/L
Additional information
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