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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
November 1999
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study not in GLP, purity of the sample has not been assessed, but the study is conducting following official guideline and scientifically valid to assessing the end point
Qualifier:
according to
Guideline:
EU Method C.3 (Algal Inhibition test)
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
no
Analytical monitoring:
no
Details on sampling:
- Sample storage conditions before analysis:internal laboratory storage
Vehicle:
no
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Source : culture collection of Algae and protozoa-Cumbria U.D. CCAP n° 27814 cultured in the internal lab
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h
Test temperature:
25°C +/-2°C
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
ca. 73 729 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Concentration Optical density after 72h Mean % Inhibition
A B C
Control 1.02 1.03 1.01 1.02  - 
WSF 0.001% 1.02 1.02 1.02 1.02 0.97
WSF 0.01% 1.02 1.01 1.00 1.01 1.76
WSF 0.1% 1.01 1.00 0.99 1.00 2.01
WSF 1% 0.85 0.85 0.84 0.85 12.68
WSF 10% 0.31 0.3 0.32 0.31 66.54
Conclusions:
After an incubation of 72 hours , there is a 50% growth inibithion of Selenastrum capricornum for 73729 mg/L of substance
Executive summary:

Fatty acid, rape oil, methyl ester has been tested for toxicity to aquatic algae according to OECD 201, on selenastrum capricornutum. Behaviour of algae is similar to that of bacteria, and Water Soluble Fractions has been prepared starting from WAF 10%.

Description of key information

Key value for chemical safety assessment

EC50 for freshwater algae:
73 729 mg/L

Additional information