Fatty acids, C14-18 and C16-18-unsatd., Me esters

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 1999

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test not GLP, purity of test material not defined, deviation from standard method due to the low solubility of the substance. the study is scientifically valid and well support the end point

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

Water Accomodated Fraction

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

WAF: Water Accomodated Fraction (ISO 147):
The solutions have to be prepared just before the test. Together with the blank, a series of solutions with concentration at a geometric series, to obtain 100% mortality after 48h and intermediate concentration to obtain a realistic EC50 at 48h.

The protocol ISO 147 has been followed, saturated solutions have been prepared at different concentrations. After 24 stirring, the fraction is stabilised for 4 hours and only the fraction with stables solution and emultion is used for the test. Since the toxicity could be generated both by the soluble fraction and the emulsion, a filtration is not appropriate.
The following solutions has been preparted: 10%, 1%, 0.1%, 0.01% and 0.001%.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Source:U.G Université de Gand
- Lenght: 2 +/- 1cm
- Feeding:no feed during test
- Frequency:daily (before testing)

ACCLIMATION
- Acclimation period:2 weeks

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

21-25°C

Details on test conditions:

TEST SYSTEM

- Aeration: no aeration

- Light intensity:12-16h daylight

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

>= 100 000 mg/L

Nominal / measured:

nominal

Conc. based on:

other: WAF: Water Accomodated Fraction

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Results:

WAF concentration	Mortality
	24h	48%	%48h
Control	0	0	0
WAF 10%	0	0	0
WAF 1%	0	0	0
WAF 0.1%	0	0	0
WAF 0.01%	0	0	0
WAF 0.001%	0	0	0

Validity criteria fulfilled:

not specified

Conclusions:

WAF EC50 48h Brachydario rerio >= 100000 mg/l

Executive summary:

The short term toxicity to fish has been assessed following OECD 203 on rape oil methyl ester, with deviation for poor soluble substances following ISO 147. No acute toxicity at the tested concentration has been revealed

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 000 mg/L