Registration Dossier

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1999
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study not in GLP, purity of the sample has not been assessed, but the study is conducting following official guideline and scientifically valid in assessing the end point
Qualifier:
equivalent or similar to guideline
Guideline:
other: ISO 10712
Deviations:
not specified
GLP compliance:
no
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
The sample is poor water soluble: the protocol ISO 147 has been followed. Saturated solutions have been prepared, the more concentrated at 10% (10g/100ml).
Starting from that, following dilution have been prepared: 8%, 1%, 0.1%, 0.01%, 0.001%
Test organisms (species):
Pseudomonas putida
Details on inoculum:
-Age of the culture stock: 7 days
-Age of pre-colture: 5+/- h at 25°C
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
16 h
Key result
Duration:
16 h
Dose descriptor:
EC0
Effect conc.:
ca. 5 250 mg/L
Nominal / measured:
nominal
Conc. based on:
other: WSF: Water Soluble Fraction
Basis for effect:
growth inhibition
Reported statistics and error estimates:
Calculation mehtod: Trimmed Spearman-Karber method

Concentration Absorbtion 0h Absorption 16h Difference % Inhibition
Control 0.015 0.101 0.086  - 
WSF 8% 0.015 0.088 0.073 15
WSF 1% 0.01 0.093 0.08 3
WSF 0.1% 0.01 0.10 0.089 0
WSF 0.01% 0.01 0.097 0.087 0
WSF 0.001% 0.01 0.099 0.089 0
Conclusions:
WSF EC0 16h Pseudonomas putida = 5250 mg/l
Executive summary:

The fatty acid, rape oil, methyl ester has been tested for The inhibition of the growth of Pseudonomas Putida following ISO 10712 and ISO 147 for poorly soluble substances

Description of key information

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
5 250 mg/L

Additional information

Categories Display