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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Remarks:
Similar to Draize-method.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1973
Report date:
1973

Materials and methods

Test guideline
Guideline:
other: similar to Draize method
Principles of method if other than guideline:
other: application: 0.1 ml, 6 albino rabbits; scoring in accordance with Draize-method.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Methacrylic acid
EC Number:
201-204-4
EC Name:
Methacrylic acid
Cas Number:
79-41-4
Molecular formula:
C4H6O2
IUPAC Name:
methacrylic acid
Test material form:
liquid
Specific details on test material used for the study:
Supplier: Rohm & Haas

Test animals / tissue source

Species:
rabbit
Strain:
other: Albino

Test system

Vehicle:
unchanged (no vehicle)
Controls:
yes, concurrent no treatment
Amount / concentration applied:
0.1 ml
Duration of treatment / exposure:
not specified
Observation period (in vivo):
1 hr, 1 days, 2 day, 3 days, 4 days, 7 days
Number of animals or in vitro replicates:
6

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
animal: #1, #2, #3, #4, #5, #6
Time point:
24/48/72 h
Score:
4
Max. score:
4
Reversibility:
not reversible
Remarks:
within 7 days
Remarks on result:
probability of severe irritation
Irritation parameter:
iris score
Basis:
animal: #1, #2, #3, #4, #5, #6
Time point:
24/48/72 h
Score:
2
Max. score:
2
Reversibility:
not reversible
Remarks:
within 7 days
Remarks on result:
probability of severe irritation
Irritation parameter:
conjunctivae score
Basis:
animal: #1, #2, #3, #4, #5, #6
Time point:
24/48/72 h
Score:
3
Max. score:
3
Reversibility:
not reversible
Remarks:
within 7 days
Remarks on result:
probability of severe irritation
Irritation parameter:
chemosis score
Basis:
animal: #2, #4
Time point:
24/48/72 h
Score:
3.33
Max. score:
4
Reversibility:
not reversible
Remarks:
within 7 days
Remarks on result:
probability of severe irritation
Irritation parameter:
chemosis score
Basis:
animal: #1, #3, #5, #6
Time point:
24/48/72 h
Score:
3
Max. score:
4
Reversibility:
not reversible
Remarks:
within 7 days
Remarks on result:
probability of severe irritation
Irritant / corrosive response data:
severe corneal, iridial and conjunctival effects persisting through the 7th day.

Applicant's summary and conclusion

Interpretation of results:
Category 1 (irreversible effects on the eye) based on GHS criteria
Executive summary:

In a study similar to Draize the eyes of 6 rabbits have been treated with 0.1 ml methacrylic acid. Severe irritation has been observed in all animals after 24, 48 and 72 hours which was not reversible within 7 days. Methacrylic acid is considered to be corrosive to eyes.