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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information

No toxic effects up to the limit of solubility of the test substance in the test medium. (72 hour EC50 > 100%v/v saturated soultion; 72 hour NOEC = 100%v/v saturated soultion), OECD 201, EU Method C.3, Vryenhoef & Mullee (2010)

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Additional information

A study was performed to assess the effect of the test material on the growth of the green alga Desmodesmus subspicatus. The method followed that described in the OECD Guidelines for Testing of Chemicals (2006) No 201, "Freshwater Alga and Cyanobacteria, Growth Inhibition Test" referenced as Method C.3 of Commission Regulation (EC) No 440/2008.

Pre-study solubility work conducted indicated that it was not possible to obtain a testable solution of the test material using traditional methods of preparation e.g. ultrasonication and high shear mixing. It was therefore considered that the most appropriate method of preparation for the test material was as a saturated solution.

Following a preliminary range-finding test, Desmodesmus subspicatus was exposed to a solution of the test material at a nominal test concentration of 100% v/v saturated solution (six replicate flasks) for 72 hours, under constant illumination and shaking at a temperature of 24 ± 1°C.  Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group, using a Coulter®Multisizer Particle Counter.

Under the conditions of the study, exposure of Desmodesmus subspicatus to the test material gave EC50 values of greater than 100% v/v saturated solution and correspondingly the No Observed Effect Concentration was 100% v/v saturated solution.Therefore, under the conditions of the study there were no toxic effects up to the limit of solubility of the test material in the test medium.