methyl acrylate; methyl propenoate

Dermal absorption

Administrative data

Endpoint:

dermal absorption in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented publication

Data source

Reference

Reference Type:

secondary source

Title:

Whole-body autoradiography after systemic and topical administration of methyl acrylate in the guinea-pig.

Author:

Seutter E and Rijntes NVM

Year:

1981

Bibliographic source:

Arch. Dermatol. Res. 270: 273-284

Materials and methods

Test guideline

Qualifier:

no guideline followed

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Methyl acrylate

EC Number:

202-500-6

EC Name:

Methyl acrylate

Cas Number:

96-33-3

Molecular formula:

C4H6O2

IUPAC Name:

methyl prop-2-enoate

Details on test material:

- Name of test material (as cited in study report): Methyl acrylate
- Analytical purity: 99 % (GC)
- Supplier: Merck, Darmstadt

- Radiochemical purity (if radiolabelling): no data
- Specific activity (if radiolabelling): 0.76 mCi/mmol
- Locations of the label (if radiolabelling): Methyl (2,3-14C)-acrylate
- Supplier: Radiochemical Centre, Amersham, England

Radiolabelling:

yes

Test animals

Species:

guinea pig

Strain:

not specified

Sex:

male

Details on test animals or test system and environmental conditions:

- Weight at study initiation: 420 g

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Doses:

0.53 mmol/kg bw (corresponding to approx. 46 mg/kg bw)

No. of animals per group:

2 animals received a dose of 0.53 mmol/kg bw of the unlabeled acrylate (corresponding to approx. 46 mg/kg bw), 6 animals received the same dose of the labeled acrylate.

Control animals:

no

Details on study design:

APPLICATION OF DOSE:
For administration on the skin a polystyrene cup of 16 mm inner diameter was glued to the shaved skin of the back with Nobecutane (Astra). After a few minutes of slight pressure the rim of the cup was connected tightly with the skin. The acrylate was injected into the cup, which was immediately closed with a lid and fastened in this position with elastic adhesive bandage of 3" width, wound around the animal in such a way as not to hinder respiration.


SITE PROTECTION / USE OF RESTRAINERS FOR PREVENTING INGESTION: yes

Results and discussion

Signs and symptoms of toxicity:

not specified

Dermal irritation:

yes

Any other information on results incl. tables

After administration in a closed cup on skin (46 mg/kg bw), a slow penetration in dermis occurred and low levels were found in the kidneys and bladder within 4 hours. After penetration of the dermis, which occurred between 6 and 40 hours, a gradual increase of radioactivity occurred in the heart, liver, lungs, tear glands, and other subcutaneous tissues throughout the body. The detoxification was screened by estimating the urinary thioether content and respiratory carbon monoxide. This showed that in addition to oxidation to CO2, the binding to SH-groups was the principal means of metabolism.