Registration Dossier

Administrative data

Endpoint:
mode of degradation in actual use
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
From 01 NOV 1993 to 20 DEC 1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented research report on microbial degradation of the registration substance

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report Date:
1994

Materials and methods

Test guideline
Qualifier:
no guideline available
Guideline:
other: Research according to standard methods
Deviations:
not applicable
Principles of method if other than guideline:
Enzyme assay
GLP compliance:
no
Remarks:
research study well documented
Type of study / information:
Research on Metabolism of Dodecyldimethylamine using standard methods

Test material

Reference
Name:
Unnamed
Type:
Constituent

Results and discussion

Any other information on results incl. tables

Identification of Strain MA3

An isolate was obtained in pure culture by streaking the enrichment culture on agar plates containing mineral salts and dodecyldimethylamine.

Oxidative cleavage of C alkyl-N bond

Cells grown on dodecyldimethylamine readily oxidized dodecyldimethylamine to dimethylamine, dodecanal and dodecanoic acid.

Applicant's summary and conclusion

Executive summary:

Pseudomonas MA3 is capable to use dodecyldimethyl amine as sole carbon and energy source oxidizing and cleaving the substance to dodecanal, dodecanoic acid and dimethylamine. Dodecanoic acid as a fatty acid is subsequently biodegraded by beta oxidation. The C alkyl - N bond is cleaved with help of a dehydrogenase. The metabolites dodecanal and dodecanoic acid were identified using specific enzyme inhibitors to stop further metabolism. Dimethylamine was determined by GC.

Finally the intermediate metabolites will be mineralised which means that Dodecyldimethylamine is ultimately biodegraded.