Registration Dossier

Administrative data

Endpoint:
cytotoxicity
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Scientifically acceptable publication

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
An Evaluation of the Utility of Four in Vitro Short Term Tests for Predicting the Cytotoxicity of Individual Compounds Derived from Tobacco Smoke
Author:
Curvall M, Enzell CR and Pettersson B
Year:
1984
Bibliographic source:
Cell Biol Toxicol 1: 173 - 193
Reference Type:
publication
Title:
Effects of Tobacco and Tobacco Smoke Constituents on Cell Multiplication in Vitro
Author:
Pilotti A, Ancker K, Arrhenius E and Enzell C
Year:
1975
Bibliographic source:
Toxicology 5: 49 - 62
Reference Type:
publication
Title:
Effect of Tobacco Smoke Compounds on the Plasma Membrane of Cultured Human Lung Fibroblasts
Author:
Thelestam M, Curvall M and Enzell CR
Year:
1980
Bibliographic source:
Toxicology 15: 203 - 217
Reference Type:
publication
Title:
Effects of Tobacco Smoke Compounds on the Cilliary Activity of the Embryo Chicken Trachea in Vitro
Author:
Petterson B, Curvall M and Enzell CR
Year:
1982
Bibliographic source:
Toxicology 23: 41-55

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Test substance cytotoxicity was accessed by evaluating its capacity to inhibit culture growth in ascites sarcoma BP8 cells, to inhibit of the oxidative metabolism in isolated brown fat cells, to increase the permeability of the membranes in human lung fibroblasts, or to increase the time to ciliostasis in the tracheas of unbom chickens.
GLP compliance:
no
Type of method:
in vitro
Endpoint addressed:
not applicable

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): p-anisaldehyde
No further details provided

Results and discussion

Any other information on results incl. tables

Cytotoxicity values for the test substance were obtained in four in vitro short term test systems. The mean activity of the results from the four test systems in a 10-point scale was determined as 0.8 (1 for cell growth inhibition in Ascites sarcoma BP 8 cells, 2 for inhibition of the oxidative metabolism in hamster brown fat cells, 0 for membrane damage of human diploid embryonic lung fibroblasts, 0 for inhibition of ciliary activity using chicken embryo trachea).

Applicant's summary and conclusion