Anisaldehyde

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Anisaldehyde

EC Number:

204-602-6

EC Name:

Anisaldehyde

Cas Number:

123-11-5

Molecular formula:

C8 H8 O2

IUPAC Name:

4-methoxybenzaldehyde

Details on test material:

- Name of test material (as cited in study report): Anisaldehyd
- Physical state: liquid
- Analytical purity: > 99 % (data from order sheet)

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Hagemann
- Mean weight at study initiation per test group: 170-260 g (males), 170-190 g (females)
- Fasting period before study: 15-20 h
- Diet: Herilan MRH-Haltung, H. Eggersmann KG; ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

CMC (carboxymethyl cellulose)

Remarks:

aqueous 0.5% CMC solution

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 4.64 - 50%

MAXIMUM DOSE VOLUME APPLIED: 10 mL/kg

Doses:

464, 2150, 3160, 3830, 5000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

other: not applicable

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Weighing was performed at the beginning of the study and on days 4, 7 and 13. Observation of clinical signs was done several times on the day of administration and once daily afterwards with the exception of weekends and on holidays
- Necropsy of survivors performed: yes

Statistics:

According to Finney, D.J.: Probit Analysis, Cambridge University Press, 3rd ed., 1971

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

3 210 mg/kg bw

Based on:

test mat.

95% CL:

2 755 - 3 600

Mortality:

5000 mg/kg bw: 10/10 animals (5/5 males died within one day; 4/5 females died within one day, the last female within 7 days)
3830 mg/kg bw: 7/10 animals (2/5 males died within 7 days; 5/5 females died within one day)
3160 mg/kg bw: 6/10 animals (2/5 males died within one day; 4/5 females died within one day)
2150 mg/kg bw: 0/10 animals
464 mg/kg bw: 0/10 animals

Clinical signs:

other: 5000 mg/kg bw: dyspnoea, apathy, abnormal position, staggering, atonia, narcotic-like behaviour, missing pain reflex, twitching, skin redness, ruffled fur, exsiccosis and poor general state before exitus 3830 mg/kg bw: dyspnoea, apathy, abnormal positi

Gross pathology:

Animals that died: dilatation of the urinary bladder, oedemateous liver parenchym, single hemorrhagic erosions of the gastreous mucosa
Sacrificed animals: no abnormalities