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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2009-05-18 to 2009-05-22
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes
Remarks:
no certificate
Specific details on test material used for the study:
TEST MATERIAL
- Name of test material (as cited in study report): decanal, Ald C-10 BHA
- Substance type: Colourless to pale yellow liquid
- Physical state: Liquid
- Purity test date: 2009-12-07
- Lot No: 0002825582
- Expiration date of the lot/batch: 2010-12
- Stability under test conditions: Stable under normal storage conditions
- Storage condition of test material: Room temperature

STABILISER CONTROL
- Name of test material (as cited in study report): BHA, 2-t-butyl-4-methoxy phenol
- Substance type: No data
- Physical state: No data
- Analytical purity: 99.9 %
- Impurities (identity and concentrations): No data
- Composition of test material, percentage of components: No data
- Isomers composition: No data
- Purity test date: 2009-12-07
- Lot No: 0000231975
- Expiration date of the lot/batch: 2010-12
- Stability under test conditions: No data
- Storage condition of test material: No data
Analytical monitoring:
yes
Details on sampling:
- Sampling time: 0, 72 hrs (freshly prepared test solutions) 24, 96 hrs (spent test solutions)
- Sampling method: 9 mL removed using pipette
- Sample storage conditions before analysis: No data
Vehicle:
no
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: Rainbow trout
- Strain: No data
- Source: Trout Lodge, Sumner, Washington, United States
- Age at study initiation (mean and range, SD): No data
- Length at study initiation (representative sample of 10): total length 46 mm, range 41 to 49 mm, SD 3.9 mm
- Weight at study initiation (representative sample of 10): mean 0.8969 g, range 0.5455 g to 1.5721 g, SD 0.2882 g
- Method of breeding: No data
- Feeding during test: No

ACCLIMATION
- Acclimation period and conditions: ≥12 d in the lab, > 7 d in dilution water within ± 2 °C of test temperature (same as test).
- Type of food: Standard commercial fish food (salmon starter diet)
- Amount of food: No data
- Feeding frequency: Daily, although fish were not offered food 24 hrs prior to their use in the definitive test
- Health during acclimation: No diseases were observed nor were the fish treated for disease, and no mortalities occurred during the 48 hr period prior to definitive test initiation
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
150 mg CaCO₃/L
Test temperature:
15.5 to 17.0 °C (table 3)
pH:
7.8 to 8.4 (Table 5).
Dissolved oxygen:
8.6 to 12.5 mg/L (91 to 131 % saturation) in fresh solutions, and from 4.3 to 10.1 in spent solutions (45 to 106 % saturation) (Table 4 of full study report)
Salinity:
Conductivity: 358 μS.
Nominal and measured concentrations:
Nominal: 0 (control), 0 (stabiliser control), 1.0, 1.5, 2.2, 3.3, and 5.0 mg/L
Measured (geometric mean): 0 (control), 0 (stabiliser control), 0.478, 0.844, 1.32, 1.76, 3.77
Details on test conditions:
RANGE-FINDING TEST
- Test vessel: 10 L glass jars
- Type: Closed (covered with parafilm)
- Material, size, headspace, fill volume: 8L test or control water per vessel
- Test treatment solutions were prepared by diluting appropriate aliquots of test substance to a volume of 8 L with dilution water.
- The stabiliser control was prepared by weighing 0.00016 g of BHA into a 10 L glass jar and bringing the jar to a volume of 8 L with dilution water
- After 48 hr, surviving fish were transferred to freshly prepared solutions.

TEST SYSTEM
- Test vessel: 21 L glass jars
- Type: Closed
- Material, size, headspace, fill volume: 21 L test or control, no headspace due to volatility
- After 48 hr, surviving fish were transferred to freshly prepared solutions.
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per stabiliser control (replicates): 1
- Biomass loading rate (instantaneous): 0.2990 g/L/day

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Moderately hard freshwater prepared blending naturally hard well water with well water that was demineralised by reverse osmosis (RO). The well water and RO water were blended together to yield a total hardness of 130 to 160 mg/L as CaCO₃.

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 hrs light / 8 hrs dark
- Light intensity: 581 lux

EFFECT PARAMETERS MEASURED: Mortality at 24, 48, 72, 96 hrs.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.5 ×
- Range finding study
- Test concentrations: 0 (control), 1.3, 2.5, 5.0, 10, and 20 mg/L
- Results used to determine the conditions for the definitive study:
-- The control and all test substance solutions < 10 mg/L were clear and colourless with no visible signs of undissolved test substance, precipitate, or surface film throughout the study.
-- The 10 and 20 mg/L test solutions had a slight surface film at initiation and 24 hrs.
-- Fish were observed after 24, 48, 72, and 96 hrs. After 96 hrs of exposure, there was no mortality or sublethal effects observed in the controls or any of the remaining test substance treatments.
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
2.1 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95 % CL 1.88 and 2.35 mg/L; Mean of measured fresh solution concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1.45 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95 % CL 1.33 and 1.59 mg
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
1.75 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Mean of measured fresh solution concentrations
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
1.32 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
- After 96 hrs of exposure, mortality was 0 %, 0 %, 0 %, 0 %, 14 %, 100 %, and 100 % in the 0 (control), 0 (stabiliser control), 0.478, 0.844, 1.32, 1.76, and 3.77 mg/L treatments respectively.
- There were no sublethal effects noted in the control or treatments during the definitive test.

Validity criteria fulfilled:
no
Remarks:
the dissolved oxygen concentration was not at least 60% of the air saturation value throughout the test (i.e. < 60% saturation was measured in the old solutions at 24 and 48 hours)
Conclusions:
The acute toxicity of decanal to rainbow trout was determined according to OECD guideline 203. The 96 hr LC₅₀ was determined to be 1.45 mg/L (based on geometric mean measured concentrations).

Although the study did not meet the guideline criterion for the dissolved oxygen concentration being >= 60% throughout the test, the study is considered valid as no mortality was observed in the two controls, both of which had similar low measured oxygen concentrations to the test solutions in the spent solutions at 24 and 48 hour.

Description of key information

Rainbow trout 96 hr LC₅₀ 1.45 mg/L (OECD 203)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Dose descriptor:
LC50
Effect concentration:
1.45 mg/L

Additional information

The acute toxicity to rainbow trout was determined according to OECD guideline 203. The 96 hr LC₅₀ was determined to be 1.45 mg/L (based on geometric mean measured concentrations).