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Short-term toxicity to aquatic invertebrates

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short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
week 43-44 2012
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Physico-Chemical properties
-Water solubility: 2.59-2.96 g/l
Analytical monitoring:
Details on sampling:
For verification of test concentrations, samples were collected from additional test beakers without animals made for this porpose only. At the initiation of the test, before and after renewall at 24 hours and at the termination of the test, duplicate subsamples were collected of each test concentration.
Details on test solutions:
A stock solution of 100 mg/l were prepared by weighing out 100 mg of the test product and dissolve it in 1 l test medium. pH were adjusted to 7.8 ± 0.2 in the stock solution. The test solutions were prepared by diluting the stock solution in the test medium. THe following nominel concentrations were tested: 0 (control); 2.0; 5.0; 10; 20; 50 and 100 mg/l
Test organisms (species):
Daphnia magna
Details on test organisms:
A strain of freshwater crustacean Daphnia magna (Straus) Cladocera, Crustacea originally isolated from Langedam (Birkerød, Denmark), har been cultured at DHI since 1979. Animals from this culture were used in the study.
Test type:
Water media type:
Limit test:
Total exposure duration:
48 h
Nominal and measured concentrations:
The analytically determined test item concentrations in the analyzed test media samples (dilutions 1:2, 1:5, 1:10, 1:20, 1:50, 1:100 and the undiluted filtrate) amounted to 1, 2, 5, 10, 20, 50 and 100 mg/l at the start of the test .The values found at the end of the test ranged from 83 to 104% of the initially measured values. Under the test conditions the test item was stable during the test period of 48 hours. The reported biological results are related to the initially measured test item concentrations.
Key result
48 h
Dose descriptor:
Effect conc.:
60 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
The 48-hour EC50 was calculated to be 60 mg/l.
The validation of the test concentrations by chemicals analyses showed a deviation from the nominel concentration throughout the test lower than 20 per cent. Therefore results are based on the nominel concentration.

Description of key information

Hazardous to the aquatioc environment. Chronic Category 3

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
60 mg/L

Additional information

In a study conducted according to OECD guideline 202 the EC50 based on mobility of the test animals (Daphnia magna) after 48 hours was 60 mg/L (nominal measured concentration).