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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Skin irritation / corrosion

Currently viewing:

Administrative data

Endpoint:
skin irritation: in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2012
Report date:
2013

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
O-isopropyl ethylthiocarbamate
EC Number:
205-517-7
EC Name:
O-isopropyl ethylthiocarbamate
Cas Number:
141-98-0
Molecular formula:
C6H13NOS
IUPAC Name:
N-ethyl(propan-2-yloxy)carbothioamide
Test material form:
other: Liquid
Details on test material:
- Name of test material (as cited in study report): IPETC
- Physical state: Liquid
- Stability under test conditions: Stable
- Storage condition of test material:ambient

In vitro test system

Test system:
human skin model
Source species:
human
Cell type:
other: EST-1000 (CellSystems® Biotechnology GmbH)

Test system

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 30 µL IPETC were applied to the skin model with a surface area of 0.6 cm²
- Concentration (if solution): 95.7

VEHICLE
- Amount(s) applied (volume or weight with unit): no vehicle used
Duration of treatment / exposure:
20 minutes
Observation period:
n/a
Number of animals:
n/a
Details on study design:
Negative control: phosphate buffered saline (D-PBS)
Positive control: 5% aqueous sodium dodecyl sulphate (SDS)

Results and discussion

In vitro

Results
Irritation / corrosion parameter:
% tissue viability
Value:
ca. 10.2
Negative controls validity:
valid
Positive controls validity:
valid
Remarks on result:
other: Mean of tissue sample responses in percentage. Time point: 20 minutes. Reversibility not tested. Indication of a positive result.
Other effects / acceptance of results:
Summarized results (Mean±SD):

Optical density 20 minutes
Extinction# (n=3 tissues) % Extinction compared to control

Negative control(D-PBS) 0.459±0.008
IPETC (test substance) 0.047±0.039 10.2
Positive control(SDS) 0.014±0.007 3.1

Applicant's summary and conclusion

Interpretation of results:
Category 2 (irritant) based on GHS criteria
Remarks:
Based on EU GHS criteria
Conclusions:
Under the present test conditions IPETC , tested at an exposure time of 20 minutes, was cytotoxic and, hence, irritant to skin in an experiment employing an artificial three-dimensional model of human skin.
Executive summary:

An In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method was performed with the test substance in accordance with OECD Guideline 439 (employing an epidermal human Skin Test 1000 (EST-1000) cell tissue system). Under the test conditions, the test substance tested at an exposure time of 20 minutes, in an artificial three-dimensional model of human skin, was cytotoxic and, hence, irritant to skin in an experiment employing an artificial three-dimensional model of human skin.