(carboxymethyl)dimethyl-3-[(1-oxododecyl)amino]propylammonium hydroxide

Toxicity to soil macroorganisms except arthropods

Description of key information

Two acute toxicity studies towards the earthworm Eisenia fetida are available.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:

846 mg/kg soil dw

Additional information

Toxicity to soil macroorganisms

The acute toxicity of C8-18 AAPB towards the earthworm Eisenia fetida was investigated in a study conducted according to OECD Guideline 207 (Earthworm, Acute Toxicity Tests) and EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test). At total 40 animals per concentration step were exposed to nominal concentrations of 0 (control), 63, 125, 250, 500, and 1000 mg product/kg dry soil for 14 d. No mortality and no effects on body weight were observed even in the highest concentration tested (14 d LC₀≥ 1000 mg product/kg dry soil; 14 d LC₀≥ 380 mg a. i /kg dry soil assuming an active matter content of 38 %). Moreover, no abnormal behaviour of the test organisms or other symptoms of toxicity were recorded in worms at any of the test treatments.

A second study, conducted as limit test, the acute toxicity of Coco AAPB towards Eisenia fetida was investigated according to EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test). 40 Animals were exposed to 1000 mg/kg dry residue of test material (sole concentration tested) over a 14-d period. The 14 d LC₀was determined to be ≥ 1000 mg dry residue/kg soil dry weight (corresponding to ≥ 846 mg active matter/kg soil dry weight and ≥ 2857 mg product/kg soil dry weight.

As there were no effects observed in both studies, it is justified to use the LC₀of 846 mg/kg soil dw for chemical safety assessment. The obtained results were considered to be valid for the AAPBs.