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Short-term toxicity to fish

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Description of key information

Reliable results on the acute toxicity of C8-18 AAPB and Coco AAPB and the prolonged toxicity of C8-18 AAPB to fresh water fish obtained from guideline studies are available. For marine fish a limit test conducted according to guideline is available.

Key value for chemical safety assessment

LC50 for freshwater fish:
1.11 mg/L
LC50 for marine water fish:
1.1 mg/L

Additional information

Freshwater fish

In a semi-static test conducted according to OECD Guideline 203 and EU Method C.1 using Pimephales promelas as test organism a 96 h LC50=1.11 mg a. i./L (nominal; no analytical monitoring of test substance concentration; pH not adjusted; pH range: 7.2 -7.6) was determined. In two semistatic test conducted according to OECD Guideline 203 and/or EC Method C.1 with Danio rerio (pH adjusted) and Cyprinus carpio (pH not adjusted; pH range: 7.8 -8.2) 96 h LC50values of ca. 2 mg a. i./L nominal were derived. Analytical monitoring of test substance concentrations were not conducted in the test with Danio rerio. In the semi-static test with Cyprinus carpio the concentration of Coco AAPB was stable during the incubation time which has been shown by analytical monitoring via TOC (non-specific method).

 

The acute toxicity of C8-18 AAPB towards fish (Danio rerio) was investigated in a study conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test) and ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae) ] - Part 1: Static Method). The 96 h LC50was found to be 15 mg/L nominal (no information whether this value refers to test material or active matter).

Furthermore the results of a prolonged fish toxicity test are available. In this 28-d toxicity study conducted according to OECD Guideline 204, rainbow trouts (Oncorhynchus mykiss) were exposed to C8-18 AAPB under flow-through conditions. The 28-d NOEC and LOEC values were 0.16 and 0.5 mg a. i./L nominal based on mortality and sub-lethal effects.

 

Saltwater fish

The acute toxicity of Coco AAPB to the marine fish Cyprinodon variegatus was investigated in a study conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test) adapted by OSPARCOM (2005 -11) for marine testing of offshore chemicals. In this limit test conducted under semi-static conditions, the fish were exposed to a single concentration of 3 mg product/L nominal (corresponding to 1.1 mg a. i./L) for 96 h. No effects were observed at 1.1 mg a. i./L nominal.

 

Conclusion

For freshwater fish (Pimephales promelas) the lowest 96 h LC50was determined to 1.11 mg a. i./L nominal, and for marine fish (Cyprinodon variegatus) to 1.1 mg a. i./L nominal.

The obtained results were considered to be valid for the AAPBs.