Alcohols, C12-18, ethoxylated

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

11 JAN - 02 FEB 1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP-Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

61791-28-4

EC Number:

612-400-8

Cas Number:

61791-28-4

IUPAC Name:

61791-28-4

Details on test material:

- Substance type: Fatty alcohol ethoxylate with 10 EO
- Analytical purity: 99.7% a.s.

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

- Concentrations: 0 ppm, 30 ppm, 100 ppm, 300 ppm, 1000 ppm

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

- Method of application to soil surface (if used): 5 g test substance were mixed with 95 g soil and dilutions of 3 : 10 and 1 : 10 were made. 10 g of each dilution were cooled by liquid air and together with deep frozen soil (150 g / vessel / concentration) homogenized. In a next step, the soil was mixed with the remaining amount of soil of 508 g.

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: earthworm
- Source: Dr. Edwards (EG-Ringtest 1983), than own breeding
- Age at test initiation (mean and range, SD): 2 month
- Weight at test initiation (mean and range, SD): 300 - 600 mg


ACCLIMATION
- Acclimation period: 24 h
- Acclimation conditions (same as test or not): same as test
- Health during acclimation (any mortality observed): no mortality

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Test conditions

Test temperature:

20 +/- 2 °C

Moisture:

The water content was adjusted 7 days prior to the test start to 35.02% of the basic substrate (= 25.93% soil moisture)

Details on test conditions:

TEST SYSTEM
- Test container (material, size): glass vessels (1 - 2 L)
- Amount of soil or substrate: 658 g/vessel
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4



SOURCE AND PROPERTIES OF SUBSTRATE (if soil): artificial soil according to the UBA guideline (March 1984)

Nominal and measured concentrations:

Nominal: 0, 30, 100, 300 and 1000 ppm

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: no mortalities at the highest concentration of 1000 ppm

Details on results:

- Mortality at end of exposure period: none
- Total mass of adults at beginning of test: since all worms survived at 1000 ppm and increase their body weight, only one replicat of the concentrations 30, 100 and 300 ppm was evaluated. 0 ppm: 9.48 g (start) / 9.51 g (end); 30 ppm: 2.5 g / 2.76 g; 100 ppm: 2.6 g / 2.64 g; 300 ppm: 2.33 g / 2.37 g; 1000 ppm: 9.28 g/ 9.75 g
- Changes in body weigth of live adults (% of initial weight) at end of exposure period: 0 ppm: increase of body weight by 3.16%; 30 ppm: increase of body weight by 10.14%; 100 ppm: increase of body weight by 1.53%; 300 ppm: increase of body weight by 1.71%; 1000 ppm: increase of body weight by 5.06%.
- No. of offspring produced: not investigated
- No. of unhatched cocoons: not investigated
- Morphological abnormalities: not investigated
- Behavioural abnormalities: not investigated
- Other biological observations: none