Registration Dossier

Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented publication which meets basic scientific principles

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
2000
Report Date:
2000

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Fish were exposed in a flow-through system to the test substance during an uptake phase of up to 72 h. The following elimination phase lasted for 24 h.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): C12EO8, C14EO4, C16EO8
- Analytical purity: > 97%
Radiolabelling:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pimephales promelas
Details on test organisms:
TEST ORGANISM
- Common name: fathead minnow
- Source: hatchery of Utrecht University, The Netherlands
- Weight at study initiation (mean and range, SD): 0.5 - 1.0 g

ACCLIMATION
- Acclimation period: at least 1 week
- Type and amount of food: Lapis dry feed at a rate of 1% of body weight
- Feeding frequency: daily

Study design

Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
54 - 72 h
Total depuration duration:
24 h

Test conditions

Test temperature:
20.7 - 22.5 °C
Details on test conditions:
TEST SYSTEM
- Test vessel: 5 - 8 L aquaria
- No. of organisms per vessel: 30


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater was prepared by dissolving 0.75 mM CaCl2 x 2H2O, 0.46 mM MgSO4 x 7H2O, 1.51 mM NaHCO3, 0.04 mM KH2PO4, 1.01 mM NaNO3 and 0.10 mM Na2SiO3 in distilled water.

Nominal and measured concentrations:
The AE concentrations were higher than the critical micelle concentration. 0.029 µM steady state concentration of the test compound in water
Reference substance (positive control):
no

Results and discussion

Lipid content
Lipid content:
0.9 - 8.2 %
Time point:
start of exposure
Remarks on result:
other: average content: 4.9%
Bioaccumulation factoropen allclose all
Type:
BCF
Value:
12.7 L/kg
Basis:
not specified
Time of plateau:
24 h
Calculation basis:
steady state
Remarks on result:
other: for C12EO8
Remarks:
Conc.in environment / dose:0.570 µM steady state concentration of the test compound in water
Type:
BCF
Value:
237 L/kg
Basis:
not specified
Time of plateau:
24 h
Calculation basis:
steady state
Remarks on result:
other: for C14EO4
Remarks:
Conc.in environment / dose:0.029 µM steady state concentration of the test compound in water
Type:
BCF
Value:
387.5 L/kg
Basis:
not specified
Time of plateau:
24 h
Calculation basis:
steady state
Remarks on result:
other: for C16EO8
Remarks:
Conc.in environment / dose:0.033 µM steady state concentration of the test compound in water
Details on results:
- Mortality of test organisms: no mortality was observed
- Behavioural abnormalities: no signs of sublethal effects, such as swimming or breathing behavior, were observed during the experiments.

Any other information on results incl. tables

The test substance was not stored inside fish and no relationship between BCF and lipid content was observed. The elimination rate suggested rapid biotransformation of the test substance.

Applicant's summary and conclusion