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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1956-57 and 1981
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non GLP, but sufficient data is available for interpetation of results.
Cross-referenceopen allclose all
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1957
Reference Type:
study report
Title:
Unnamed
Year:
1981
Reference Type:
other company data
Title:
Unnamed
Year:
1956
Reference Type:
study report
Title:
Unnamed
Year:
1956

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Female rats were exposed to graded doses of 1-nitropropane vapor in a closed chamber for a periods of one hour. The LC50 concentrations were determined.
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
1-Nitropropane :
Purity: 96.12%
Source: Sterington, Lousianna
Lot number: 9A17-7B

Test animals

Species:
rat
Strain:
Wistar
Sex:
female
Details on test animals and environmental conditions:
Six female Wistar rats weighing 150 - 200 g.

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: unchanged (no vehicle)
Details on inhalation exposure:
One hour inhalation exposures were conducted to determine the LD50 of the compound. The atmospheres were generated using a constant infusion pump and a vaporizer. The exposures occurred under dynamic airflow conditions.
Analytical verification of test atmosphere concentrations:
yes
Remarks:
Colormetric analysis
Duration of exposure:
1 h
Concentrations:
Actual concentrations: 8.60, 11.02, and 14.40 mg/L
No. of animals per sex per dose:
6 female rats per dose
Control animals:
not specified
Details on study design:
Female rats were exposed to graded doses of 1-nitropropane vapor in a closed chamber. One hour inhalation exposures were conducted to determine the LC50 of the compound. The atmospheres were generated using a constant infusion pump and a vaporizer. The exposures occurred under dynamic airflow conditions. Animals were observed 7 days following exposure.
Statistics:
The following endpoint was established using the Miller-Tainter method of LD50 dosage response determination.


Results and discussion

Preliminary study:
No data
Effect levels
Sex:
female
Dose descriptor:
LC50
Effect level:
11.02 mg/L air
Exp. duration:
1 h
Mortality:
Mortality observed in animals over 7 day period following exposures to 8.6, 11.02, and 14.40 mg/L where 0, 3, and 5 animals died after one hour of exposure, respectively.
Clinical signs:
Irritation at five minutes of exposure, no convulsant action noted except at 17 mg/L were it appeared slight. Concentration of 1-nitropropane was not sufficient for anesthetic effects. All animals exhibited ataxia following removal from the chamber. Normal movement became apparent after 24 hours.
Body weight:
No data
Gross pathology:
No data
Other findings:
None

Any other information on results incl. tables

The acute inhalation toxicity, the LC50 was calculated from a rat LC50 at 1 hr (11.02 mg/L) by dividing by 2 as per the following guideline, "NFPA, Recommendations on the Transport of Dangerous Goods, UN Publication, 4th Ed. Section 6.4)." The calculated LC50 value of 5.5 mg/L was converted to 5500 mg/m3.

Applicant's summary and conclusion

Interpretation of results:
Toxicity Category III
Remarks:
Migrated information Criteria used for interpretation of results: other: EU GHS
Conclusions:
Following a 1 hour inhalation exposure, the LC50 is 11.02 mg/l .
Executive summary:

None