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Short-term toxicity to aquatic invertebrates

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Description of key information

No toxic effects up to the limit of water solubility for Daphnia magna (EU Method C.2)

Key value for chemical safety assessment

Additional information

There are no studies available on the toxicity of isopentyl laurate (CAS No. 6309-51-9) on aquatic invertebrate. The assessment of short-term toxicity was therefore based on studies conducted with the structurally most similar category members, Fatty acids, C8-16, 2-ethylhexyl esters (CAS No. 135800-37-2) and isopropyl myristate (CAS No. 110-27-0). This read across approach is in accordance with Regulation (EC) No 1907/2006, Annex XI, 1.5. Grouping of substance and read across approach. Further justification is given within the endpoint summary 6.1 and within the category justification in Section 13. In this case of read-across, the best suited (highest degree of structural similarity, nearest physico-chemical properties) read-across substance was entered into IUCLID. Nevertheless, as can be seen in the data matrix of the category justification in Section 13, all reliable data in the category support the hazard assessment for this endpoint, by showing a consistent pattern of results.

The key study with Fatty acids, C8-16, 2-ethylhexyl esters (CAS No. 135800-37-2) was performed as a limit test, according to EU Method C.2 (Wierich, 1998). The test organism Daphnia magna was exposed to the test substance in a static system for 48 hours. Three different methods were used for the preparation of test solutions: 1) direct addition to 100 mg/L, 2) direct addition to 100 mg/L with removal of undissolved test substance and 3) 3-5 times saturation without separation of undissolved material. With the first treatment daphnids got stuck to oil drops on the surface or to the walls of the vessel. With the second and third treatments no immobilisation was observed, and an EC50 of > 100 mg/L is reported.

The key study with isopropyl myristate (CAS No. 110-27-0) was performed as a limit test according to EU Method C.2 and GLP (Stelter, 1995). The test organism Daphnia magna was exposed to the test substance in a static system for 48 hours. Three different methods were used for the preparation of test solutions: 1) direct addition to 100 mg/L, 2) direct addition to 100 mg/L with removal of undissolved test substance and 3) 3-5 times saturation without separation of undissolved material. With the first method an oil film was observed at the surface, and up to 100% immobilisation occurred. With the other two methods, no significant effect was observed. Based on the results, the observed immobilisation is most probably due to physical effects caused by undissolved test substance. It can therefore be concluded that the test substance had no significant toxic effect on the test organism up to the limit of water solubility. An EC50 of > 0.05 mg/L was reported.

Thus, based on theabove mentioned results, and due to the structural and profile similarities of the substances, as are explained within the overall endpoint summary 6.1 it can be concluded that no toxicological effects on aquatic invertebrates are expected up tothe limit of water solubility for isopentyl laurate (CAS No. 6309-51-9).