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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guideline
no guideline followed
Principles of method if other than guideline:
Acute toxicity upon intraperioneal injection within a 14-observation period.
GLP compliance:
Limit test:

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethyl phenyl(2,4,6-trimethylbenzoyl)phosphinate
EC Number:
EC Name:
Ethyl phenyl(2,4,6-trimethylbenzoyl)phosphinate
Cas Number:
Molecular formula:
ethyl phenyl(2,4,6-trimethylbenzoyl)phosphinate
Details on test material:
- Name of test material (as cited in study report): Initiator 654
- Physical state: liquid
- Expiration date of the lot/batch: Feb. 1983

Test animals

Details on test animals or test system and environmental conditions:
Acclimatization period: Acclimatization in the animal care unit for at least one week.
Age of animals at beginning of study: About 12 weeks
Type of cage: Type DK-III stainless steel wire mesh cages (supplied by Becker & Co., Castrop-Rauxel, FRG)
Number of animals per cage: 5
Animal identification: Identification groups using cage cards
Room temperature: 20 - 26°C
Relative humidity: 45 - 75%
Change of air: 15 - 20 times/hour
Day/night rhythm: 12 h/12 h (6.00 - 18 00 hours/18.00 - 6.00 hours)
Drinking water: Fully demineralized water each workday, ad libitum; tap water on public holidays, ad libitum
Diet: SSNIFF R; Ssniff Versuchstierdiäten, Soest, FRG
Fasting period: 16 hours before administration, water available ad libitum

Administration / exposure

Route of administration:
CMC (carboxymethyl cellulose)
0.5% in water
Details on exposure:
Preparation with 0.5% aqueous carboxymethyl cellulose
Reason for the vehicle: Aqueous formulation corresponds to the physiological medium
Form of administration: Emulsion
Application volume: 10 ml/kg
215, 681, 1000, 1470, 2000 mg/kg bw
No. of animals per sex per dose:
Control animals:
Details on study design:
Observation period: 14 days
Cageside observation: Recording of signs and symptoms < 15', 15', 30', 1 h, 2 h, 4 h, and 5 h after administration of test substance and then once each workday. Check for moribund and dead animals twice each workday and once daily at weekends and on public holidays.
Pathology: Withdrawal of feed 16 hours before sacrifice with CO2, followed by necropsy and gross-pathological examination. All animals that died were necropsied as early as possible.

Results and discussion

Effect levels
Dose descriptor:
Effect level:
ca. 1 470 mg/kg bw
215, 681 and 1000 mg/kg: no deaths after 14 days; 1470 mg/kg: 4/10 died within 2 days; 2000 mg/kg: 10/10 died within 1 day
Clinical signs:
Male animals:
All groups showed dyspnea, apathy, staggering, and poor general state.
Animals of the 3 highest doses also showed atonia, paresis, clonic convulsions, fasciculation, and abnormal position (also oberseved in animals treated with 681mg/kg)
Furthermore, high dose animals exhibited stertor, spasmodic respiration, absent pain reflex, narcotic like state, exsiccosis, and salivation (also in the 1470mg/kg group)
Additionally, trembling, twitching, tonus with stretching or bending, straub reaction, piloerection, erythema, and disequilibrium were oberserved in some of the dose groups.

Female animals:
All groups showed dyspnea, apathy, staggering, and poor general state.
All except low dose animals exhibited atonia, paresis, abnormal position.
Fasciculation and salivation were observed in the 3 highest doses.
In high dose females spasmodic respiration, absent pain reflex, narcotic like state, trembling (also animals receiving 1470mg/kg), exsiccosis, disequilibrium, clonic convulsions (also animals receiving 1470mg/kg) were also noted.
Additionally, twitching, tonus with stretching or bending, straub reaction, piloerection, erythema, exopthalamus, and cachexia were oberserved in some of the dose groups.

Clinical signs were observed for a maximum of 2 h in the two lowest dose groups, up to about 1 day in the mid dose animals, and for several days or until death in the two highest dose groups.
Body weight:
Mean body weight male animals: 194 g at study start, 280 g after 13 days
Mean body weight female animals: 188 g at study start, 230 g after 13 days
Gross pathology:
Animals that died (male & female): Intraabdominal precipitates of the test substance.
Sacrificed animals (male & female): Liver: Considerably rounded lobes and adhesions of the individual lobes to each other; liver/spleen/kidneys: milky coatings, adhesions of liver, stomach, and spleen occasionally.

Applicant's summary and conclusion