Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 282-810-6 | CAS number: 84434-11-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 5 August 2013 - 23 August 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- adopted 13th April 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 30 May 2008 (O.J. L 142 of 31.5.2008)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Chemical name Phosphinic acid, phenyl(2,4,6-trimethylbenzoyl)-,ethyl ester
Empirical formula C18H21O3P
Physical form Liquid, viscous
Water solubility Insoluble at 20°C: 35 mg/l
Purity (1H-NMR) 95.7 g/100 g
Purity (GC) 97.0 area-% (DB-1) – 97.8 area-% (DB-1701)
Test substance storage At room temperature, protected from light - Analytical monitoring:
- yes
- Details on sampling:
- HPLC analyses of the test substance concentrations was conducted in the definitive test at the beginning and after 24 and 48 h of exposure.
- Vehicle:
- no
- Details on test solutions:
- The loadings of the test material were prepared by adding the respective amounts of an acetonic stock solution to empty glass vessels. After complete evaporation of the solvent, the aerated Daphnia medium (Tables 1 and 2) was added, moderately stirred for 24 h, followed by filtration (MILLIPORE AP15 glass fiber filter). The resulting saturated solutions were used in the test.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Test organism: Daphnia magna (Straus, 1820)
Breeding: Parental and young Daphnia held in 4 l glass aquaria (3 l medium) at 20 ± 2 °C
Illumination: 16 h per day
Medium: Continuously aerated Elendt M4 medium; prepared with deionised water (conductivity <1.5 µS/cm)
Feed: Suspension of Desmodesmus subspicatus in Elendt M4 medium with an optical density OD680 of about 15 units Frequency of feeding: 400 μl per Daphnia per day, except on weekends
Control of sensitivity: Acute reference test with potassium dichromate conducted twice a year - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- Total hardness (as sum of Ca and Mg): 2.52 mmol/l
Carbonate hardness: 0.32 mmol/l - Test temperature:
- 22 °C
- pH:
- control: 7.3 - 7.4
exposure groups: 7.3 - 7.5 - Dissolved oxygen:
- control: 8.2
exposure groups: 7.5 - 8.1 - Salinity:
- Not reported - Freshwater
- Conductivity:
- Not reported - Freshwater
- Nominal and measured concentrations:
- Based on the results of the range finding test, the definitive test was performed at the following loading rates: 6.25, 12.5, 25.0, 50.0 and 100 mg/l
- Details on test conditions:
- Test vessel 100 ml flasks, all-glass, with 50 ml of test medium
Test medium Elendt M4 medium (Tables 1 and 2); prepared with deionised water (conductivity <1.5 μS/cm)
Number of Daphnia 40 individuals per test concentration, 20 per vessel
Age Less than 24 h
Light 16 h photoperiod a day, supplied by overhead white fluorescent tubes
Temperature (target) 20 ± 2 °C (temperature-controlled room)
Test type Static exposure conditions
Test duration 48 h
For each test series, the following number of test flasks was used:
Test suspension (Tn); containing test medium, test substance, two replicate samples with 20 Daphnia each
Blank control (Bn); containing test medium, three replicate samples with 20 Daphnia each - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.55 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Lower 95%-cl 6.02; Upper 95%-cl 9.67
- Duration:
- 48 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 1.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Lower 95%-cl 7.59; Upper 95%-cl 11.2
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.26 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Lower 95%-cl 11; Upper 95%-cl 14.8
- Details on results:
- HPLC analysis of Lucirin TPO-L was performed at the beginning of the exposure, after 24 h and at the end of the exposure (48 h). The analysis revealed that the test substance was only partially soluble: measured concentrations at the beginning of
the exposure were 1.10, 3.37, 8.36, 16.3 and 32.7 mg/l.
The test concentrations could satisfactorily be maintained over the whole period of exposure: geometric mean concentrations after 24 (GM0-24h) and 48 h (GM0-48h) of exposure were 1.21, 3.10, 7.52, 17.9 and 28.8 mg/l (110, 92, 90, 110 and 88% of initial concentration, respectively) and 1.00, 2.85, 6.75, 16.2 and 29.2 mg/l (90, 85, 81, 100 and 89% of initial concentration, respectively). The effective concentrations (EC10, EC20, EC50) of Lucirin TPO-L to Daphnia magna were assessed based on the geometric mean (GM) concentrations. - Results with reference substance (positive control):
- The EC50 for the control of sensitivity for 24 h of exposure with potassium dichromate was estimated to be 0.8 mg/l (data from 17 July 2013). The resulting EC50 value of the reference item was within the recommended range of 0.6 - 2.1 mg/L according to OECD Guideline 202.
- Reported statistics and error estimates:
- 95% confidence limits reported
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48 hour EC50 of the test material to Daphnia magna was determined to be 2.26 mg/l under the conditions of the test.
- Executive summary:
The effective concentrations (EC10, EC20, EC50) of Lucirin TPO-L to Daphnia magna were investigated under static exposure conditions over a period of 48 h. 40 individual Daphnia divided into 2 test vessels were exposed to the test substance.
The applied loading rates were 6.25, 12.5, 25.0, 50.0 and 100 mg/l. The loadings of the test material were prepared by adding the respective amount of an acetonic stock solution to an empty glass vessel. After complete evaporation of the solvent, the aerated Daphnia medium was added, moderately stirred for a minimum of 24 h, followed by filtration. The resulting saturated solutions were used in the test.
HPLC analysis of Lucirin TPO-L was performed at the beginning of the exposure, after 24 h and at the end of the exposure (48 h). The analysis revealed that the test substance was only partially soluble: measured concentrations at the beginning of the exposure were 1.10, 3.37, 8.36, 16.3 and 32.7 mg/l. The test concentrations could satisfactorily be maintained over the whole period of exposure: geometric mean concentrations after 24 (GM0-24h) and 48 h (GM0-48h) of exposure were 1.21, 3.10, 7.52, 17.9 and 28.8 mg/l (110, 92, 90, 110 and 88% of initial concentration, respectively) and 1.00, 2.85, 6.75, 16.2 and 29.2 mg/l (90, 85, 81, 100 and 89% of initial concentration, respectively). The effective concentrations (EC10, EC20, EC50) of Lucirin TPO-L to Daphnia magna were assessed based on the geometric mean (GM) concentrations.
The results are summarized in the following table showing ECx values and 95% confidence limits (cl):
Parameter EC10 EC20 EC50 24 h exposure GM0-24 h conc. [mg/l] 8.08 9.6 12.9 Lower 95%-cl 6.02 7.59 11 Upper 95%-cl 9.67 11.2 14.8 Chi2 0.063 Regression model: Logit Intercept a -12 Slope b 10.8 48 h of exposure GM0-48 h conc. [mg/l] 1.55 1.9 2.69 Lower 95%-cl 1.01 1.39 2.27 Upper 95%-cl 1.92 2.26 3.12 Chi2 2.13 Regression model: Logit Intercept a -3.94 Slope b 9.17
Reference
Description of key information
The study was conducted to recognised testing guidelines, and with GLP certification.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 2.26 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
