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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Study period:
1970
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The available documentation of this study, which was performed 1970, is limited. However the study is scientifically valid and the results are reliable. There is no need to repeat this vertebrate study. Read-across.
Cross-reference
Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1970
Report Date:
1970

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Six male rats were whole-body exposed to the test item for 4 hours. Analytical determination of test item concentration were performed by gas chromatography. After a 14 d observation period, animals were sacrificed and histopathologic studies were conducted.
GLP compliance:
no
Test type:
standard acute method
Limit test:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Molecular formula: C2Cl3F3 (identical to submission substance)
- Molecular weight: 187.376 g/mol (identical to submission substance)
- Smiles notation: ClC(F)(F)C(Cl)(Cl)F (different from submission substance)
- InChl: InChI=1S/C2Cl3F3/c3-1(4,6)2(5,7)8 (different from submission substance)
- Structural formula: see attachment CAS_76-13-1_Structure.png (under "Illustration (picture/graph)")
- Physical state: liquid

Test animals

Species:
rat
Strain:
other: ChR-CD
Sex:
male
Details on test animals and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 251 - 280 g

Administration / exposure

Route of administration:
inhalation: gas
Type of inhalation exposure:
whole body
Vehicle:
air
Details on inhalation exposure:
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: bell jar
- Exposure chamber volume: 16 liter
- Source and rate of air: houseline air (of test facility)
- System of generating particulates/aerosols: the test item was dispensed into a heated (65°C) stainless steel T-tube using a syringe and diluted with air to give the desired athmospheric concentration.

TEST ATMOSPHERE
- Brief description of analytical method used: GC
- Samples taken from breathing zone: no

Analytical verification of test atmosphere concentrations:
yes
Remarks:
by GC
Duration of exposure:
4 h
Concentrations:
nominal concentration: 50000 ppm v/v
analytical concentration, pure test material: 45500 ppm v/v, equivalent to 348 mg/L
analytical concentration, commercial test material: 49500 ppm v/v, equivalent to 379 mg/L
No. of animals per sex per dose:
6 male rats were treated per group
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days

Results and discussion

Effect levelsopen allclose all
Sex:
male
Dose descriptor:
LC50
Effect level:
> 348 mg/L air (analytical)
Based on:
test mat.
Exp. duration:
4 h
Remarks on result:
other: pure grade test material
Sex:
male
Dose descriptor:
LC50
Effect level:
> 379 mg/L air (analytical)
Based on:
test mat.
Exp. duration:
4 h
Remarks on result:
other: commercial grade test material
Mortality:
In each group (i.e. exposed to pure and commercial test material, respectively), one animal died after 3 h of exposure.
Clinical signs:
Clinical sighs observed during exposure were: hyperactivity, irregular respiration, pallor, uncoordinated movements, "piano-players" syndrome
Body weight:
Normal body weight gain was observed throughout the study.

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The acute inhalation toxicity of 1,1,2-trichlorotrifluoroethane (two groups: either with pure or with commercial grade test material) was tested by exposure of six male ChR-CD rats for 4 h. After a 14 d observation period, animals were sacrificed. No histopathologic studies were conducted. Clinical signs during exposure were hyperactivity, irregular respiration, uncoordinated movements, and a symptom reffered as "piano-players syndrome". Normal body weight gain was observed in the post-exposure period. In both groups (i.e. tested with pure and commercial grade test material), one of the six animals died and the LC50 was greater than the analytical concentrations of 45000 and 49000 ppm v/v, respectively.

The acute inhalation toxicity of the target chemical 1,1,1-trichloro-2,2,2-trifluoroethane is determined by read-across from an in vivo test (rats) with the source chemical 1,1,2-trichloro-1,2,2-trifluoroethane. The analogue approach is justified in Section 13 (Assessment Reports_Read-Across, in attachment CAS_354-58-5_Read-Across.pdf). As a consequence the value for LC50 (4 h, rats) of 1,1,1-trichloro-2,2,2-trifluoroethane is greater than 45000 ppm v/v (i.e. >348 mg/L air).
Executive summary:

The acute inhalation toxicity of 1,1,2-trichlorotrifluoroethane (two groups: either with pure or with commercial grade test material) was tested by exposure of six male ChR-CD rats for 4 h. After a 14 d observation period, animals were sacrificed. No histopathologic studies were conducted. Clinical signs during exposure were hyperactivity, irregular respiration, uncoordinated movements, and a symptom reffered as "piano-players syndrome". Normal body weight gain was observed in the post-exposure period. In both groups (i.e. tested with pure and commercial grade test material), one of the six animals died and the LC50 was greater than the analytical concentrations of 45000 and 49000 ppm v/v, respectively.

The acute inhalation toxicity of the target chemical 1,1,1-trichloro-2,2,2-trifluoroethane is determined by read-across from an in vivo test (rats) with the source chemical 1,1,2-trichloro-1,2,2-trifluoroethane. The analogue approach is justified in Section 13 (Assessment Reports_Read-Across, in attachment CAS_354-58-5_Read-Across.pdf). As a consequence the value for LC50 (4 h, rats) of 1,1,1-trichloro-2,2,2-trifluoroethane is greater than 45000 ppm v/v (i.e. >348 mg/L air).