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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Study period:
1962
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The available documentation of this study, which was performed 1962, is limited. However the study is scientifically valid and the results are reliable. There is no need to repeat this vertebrate study.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1962
Report Date:
1962

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
0.1 mL of the test item was applied in one eye of each of 3 rabbits. The test item was either washed out with water after 20 sec. exposure or the eyes were not washed. Clinical effects were observed after 1, 2, and 3 days after exposure.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Molecular formula: C2Cl3F3 (identical to submission substance)
- Molecular weight: 187.376 g/mol (identical to submission substance)
- Smiles notation: ClC(F)(F)C(Cl)(Cl)F (different from submission substance)
- InChl: InChI=1S/C2Cl3F3/c3-1(4,6)2(5,7)8 (different from submission substance)
- Structural formula: see attachment CAS_76-13-1_Structure.png (under "Illustration (picture/graph)")
- Physical state: liquid

Test animals / tissue source

Species:
rabbit
Strain:
other: Albino
Details on test animals or tissues and environmental conditions:
3 albino male rabbits

Test system

Vehicle:
unchanged (no vehicle)
Controls:
no
Amount / concentration applied:
0.1 mL of liquid test substance
Duration of treatment / exposure:
Group 1 (3 eyes: one eye of each animal): 20 seconds (eyes washed after exposure)
Group 2 (3 eyes: one eye of each animal): continuous (eyes not washed after exposure)
Observation period (in vivo):
up to 72 h ( i.e. 3 day) after exposure
Number of animals or in vitro replicates:
3
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Group 1: eyes of group 1 ( 3 eyes: one eye of each animal) animals were washed, time of removal after start of exposure: 20 seconds after exposure
- Group 2: no removal of test substance

Results and discussion

In vivo

Irritant / corrosive response data:
Group 1 (i.e. eyes washed 20 seconds after exposure): Mild conjunctivitis was observed in one of three animals at 24 h. Effects were completely reversible within 48 h (i.e. all eyes normal at 48 h and 72 h after eposure)
Group 2 (i.e. eyes not washed): minimal corneal dullness was observed in one of three animals at 24 h. Effects were completely reversible within 48 h (i.e. all eyes normal at 48 h and 72 h after eposure)

Any other information on results incl. tables

Group 1 (i.e. eyes washed 20 seconds after exposure): Mild conjunctivitis was observed in one of three animals at 24 h. Effects were completely reversible within 48 h (i.e. all eyes normal at 48 h and 72 h after eposure)

Group 2 (i.e. eyes not washed): minimal corneal dullness was observed in one of three animals at 24 h. Effects were completely reversible within 48 h (i.e. all eyes normal at 48 h and 72 h after eposure)

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information
Conclusions:
The eye irritation potential of the test substance 1,1,2-trichloro-1,2,2-trifluoroethane was assessed in an in vivo test on Albino rabbits. The test substance was applied to the eyes of 3 Albino rabbits per group which were either washed after 20 seconds of exposure or remained unwashed. In the animals without washing, minimal corneal dullness was observed in one of three animals at 24 h; when washed after 20 seconds, the test item caused mild conjunctivitis in one of three animals at 24 h. All effects were completely reversible within 48 h. Thus the test substance is considered to be not irritating to rabbit eyes.

The eye irritation potential of the target chemical 1,1,1-trichloro-2,2,2-trifluoroethane is determined by read-across from an in vivo test on Albino rabbits with the source chemical 1,1,2-trichloro-1,2,2-trifluoroethane. The analogue approach is justified in Section 13 (Assessment Reports_Read-Across, in attachment CAS_354-58-5_Read-Across.pdf). As a consequence 1,1,1-trichloro-2,2,2-trifluoroethane is not considered as irritating to rabbit eyes.
Executive summary:

The eye irritation potential of the test substance 1,1,2-trichloro-1,2,2-trifluoroethane was assessed in an in vivo test on Albino rabbits. The test substance was applied to the eyes of 3 Albino rabbits per group which were either washed after 20 seconds of exposure or remained unwashed. In the animals without washing, minimal corneal dullness was observed in one of three animals at 24 h; when washed after 20 seconds, the test item caused mild conjunctivitis in one of three animals at 24 h. All effects were completely reversible within 48 h. Thus the test substance is considered to be not irritating to rabbit eyes.

The eye irritation potential of the target chemical 1,1,1-trichloro-2,2,2-trifluoroethane is determined by read-across from an in vivo test on Albino rabbits with the source chemical 1,1,2-trichloro-1,2,2-trifluoroethane. The analogue approach is justified in Section 13 (Assessment Reports_Read-Across, in attachment CAS_354-58-5_Read-Across.pdf). As a consequence 1,1,1-trichloro-2,2,2-trifluoroethane is not considered as irritating to rabbit eyes.