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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The species is not the recommended one for classification and the observation period was shorter than the period recommended in the guidelines.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1941

Materials and methods

Principles of method if other than guideline:
The acute toxicity was determined for trichloroacetic acid. The mice used were young adult Albinos of a heterogeneous stock with the sexes equally distributed. Test solution was administered orally by a straight metal catheter attached to a syringe. The doses tested were 3162, 3981, 4217, 4467, 4500, 4732, 5012, 5309, 5623 and 6000 mg/kg. Immediately following the treatment the animals were observed for 6 days.
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): trichloroacetic acid

Test animals

Species:
mouse
Strain:
not specified
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Age at study initiation: Young adult

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
water
Details on oral exposure:
The acid solution before administration was adjusted with sodium hydroxide to a range of pH between 6 and 7.
Volume of administration: approximately 0.01 cc. of solution per gram of body weight.
Doses:
3162, 3981, 4217, 4467, 4500, 4732, 5012, 5309, 5623 and 6000 mg/kg
No. of animals per sex per dose:
10 animals per dose (5 males and 5 females)
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 6 days
- Other examinations performed: clinical signs, body weight, behaviour.
Statistics:
The dosage-mortality curves were constructed according to the method of Bliss.

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
4 970 mg/kg bw
95% CL:
4 700 - 5 260
Mortality:
Table of results: Doses of acid administered and numbers of fatalities resulting from each dose.
Clinical signs:
Animals receiving trichloracetic acid quickly passed into a state of narcosis or seminarcosis, and within 36 hours either recovered completely or died without coming out of the narcosis. The intoxication symptoms which occurred in mice included ataxia, sedation, prone and lateral position, diminished food uptake, decreased capability of triggering reflexes, palsy of the hind extremities and coma.

Any other information on results incl. tables

Table 07.02.01_01. Doses administered and numbers of fatalities resulting from each dose.

 

  Dose (mg/kg)

 Mortality rate

 3162

0/10 

 3981

 1/10

 4217

 1/10

 4467

 2/10

 4500

 7/10

 4732

 5/10

 5012

 3/10

 5309

 4/10

 5623

 7/10

 6000

 10/10

 

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The LD50 of trichloroacetic acid for mice was 4970 mg/kg (the acid solution before administration was adjusted with sodium hydroxide to a range of pH between 6 and 7).
Executive summary:

The acute toxicity was determined for trichloroacetic acid. The mice used were young adult Albinos of a heterogeneous stock with the sexes equally distributed. Test solution was administered orally by a straight metal catheter attached to a syringe. The doses tested were 3162, 3981, 4217, 4467, 4500, 4732, 5012, 5309, 5623 and 6000 mg/kg. Immediately following the treatment the animals were observed for 6 days. The dosage-mortality curves are constructed for each substance according to the methods of Bliss.

The LD50 of trichloroacetic acid for mice was 4970 mg/kg. Depending on the dosage strength, the experimental animals died between two hours and, at the least, three days after oral application of lethal doses. The intoxication symptoms which occurred in mice included ataxia, sedation, prone and lateral position, diminished food uptake, decreased capability of triggering reflexes, palsy of the hind extremities and coma.