Aluminum magnesium vanadium oxide

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

31 Jan - 22 Apr 2013

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Version / remarks:

2008

GLP compliance:

no

Remarks:

according to Chinese GLP (GB/T 22275.1-2008); comparable to OECD GLP C(97)186/FINAL

Analytical monitoring:

yes

Details on sampling:

- Concentrations: All concentrations, samples from frshly prepared and aged media
- Sampling method: One vessel was selected randomly and one 10 mL samples was taken
- Sample storage conditions before analysis: The samples, which were not analyzed within 24 h after sampling, were stored at 5 ± 3 °C

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Loading rates of 0.160, 0.340, 0.800, 1.70, 3.60 and 8.00 mg/L were prepared by stirring (via magnetic stirrer) in the dark at 700 rpm for 72 h. The media were then filtered by using a 0.45 μm nitrocellulose membrane. The filtrates were stored at room temperature.The filtrate was directly used as the test solution.
- Differential loading: yes

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Source: in-house culture; originally obtained from MicroBioTests Inc (ID: DM-MBT-121126)
- Age of parental stock: < 24 hours old
- Feeding during test: yes
- Food type: concentrated freshwater suspensions of unicellular green algae Desmodesmus Subspicatus and Pseudokirchneriella subcapitata
- Amount: at a rate equivalent to 0.1- 0.2 mg of organic carbon per adult per day
- Frequency: daily

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Hardness:

140 - 175 mg/L as CaCO3

Test temperature:

19.4 - 20.3 °C

pH:

7.03 - 7.90

Dissolved oxygen:

8.03 - 9.14 mg O2/L

Nominal and measured concentrations:

control, 0.160, 0.340, 0.80, 1.70, 3.60 and 8.00 mg/L (nominal, loading rate)
Measured concentrations see tables in Any other information on materials and methods field

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, fill volume: 75 mL glass vessels containing approximately 60 mL of the test medium
- Renewal rate of test solution (frequency): on days 2, 4, 7, 9, 11, 14, 16 and 18
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Culture medium different from test medium: No
- Intervals of water quality measurement: Oxygen concentration temperature, hardness and pH values were measured twice a week, in the fresh and aged test medium of the control(s) and the highest test substance concentration

OTHER TEST CONDITIONS
- Adjustment of pH: yes (to pH 7 by using 2 mol/L HCl for 10 mol/L NaOH
- Photoperiod: 16 hours light and 8 hours dark
- Light intensity: 1200 lux

EFFECT PARAMETERS MEASURED: reproduction and mortality were recorded five to seven times per week

Reference substance (positive control):

no

Key result

Duration:

21 d

Dose descriptor:

NOELR

Effect conc.:

0.16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Remarks:

loading rate

Basis for effect:

reproduction

Remarks on result:

other: (corresponding to the time-weighted mean measured concentrations of Al, V and Mg of 0.00833, 0.00213 and 8.64 mg/L)

Duration:

21 d

Dose descriptor:

LOELR

Effect conc.:

0.34 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Remarks:

loading rate

Basis for effect:

reproduction

Remarks on result:

other: (corresponding to the time-weighted mean measured concentrations of Al, V and Mg of 0.00821, 0.00320 and 8.48 mg/L);

Duration:

21 d

Dose descriptor:

EL50

Effect conc.:

3.71 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Remarks:

loading rate

Basis for effect:

reproduction

Remarks on result:

other: 95% confidence limits: was 1.83-54.4 mg/L

Table 1: Mortalities of parent animals

Day	Control	Loading rate [mg/L]
		0.160	0.340	0.800	1.70	3.60	8.00
2d	0	0	0	0	0	0	1
4d	0	0	0	0	0	0	4
7d	0	0	1	0	0	0	0
9d	0	0	0	0	0	0	0
11d	0	0	0	0	0	0	3
13d	0	0	0	0	0	0	0
17d	0	0	0	0	0	0	0
18d	0	0	0	0	0	1	1
21d	0	0	0	0	0	0	1
Cumulative adult mortalities	0	0	1	0	0	1	10
Mortality（%）	0	0	10	0	0	10	100

 

Table 2: Number live offspring:

Loading rate [mg/L]	Cumulative No. of offspring after 21 days	Parallel group
		1	2	3	4	5	6	7	8	9	10
Control	Total	107	102	98	127	94	108	101	134	93	103
0.160	Total	122	100	115	108	104	104	99	102	99	109
0.340	Total	M	88	67	50	52	68	87	70	62	60
0.800	Total	100	95	92	83	88	95	80	90	73	77
1.70	Total	71	70	65	67	58	68	73	82	59	68
3.60	Total	67	30	65	49	60	38	M	57	53	41
8.00	Total	M	M	M	M	M	M	M	M	M	M

M：Mortality of parent

 

Description of key information

NOELR (21 d) = 0.160 mg/L (nominal, loading rate) (OECD 211, Daphnia magna). Corresponding to the time-weighted mean measured concentrations of Al, V and Mg of 0.00833, 0.00213 and 8.64 mg/L, respectively

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.16 mg/L

Additional information

A semi-static test design according to OECD 211 was chosen in a study according to Chinese GLP to evaluate the chronic toxicity of aluminium magnesium vanadium oxide to freshwater invertebrates at nominal concentrations from 0.16 mg/L to 8.00 mg/L (2013). The substance has a low water solubility and therefore the test solution was prepared by adding the appropriate test item to the dilution water. After stirring for 72 hours the resulting solution was filtrated through 0.45 µm filters. Following this, the filtrates were used for the main test without dilution. The test solutions included the dilution water control and nominal concentrations of 0.160, 0.340, 0.800, 1.70, 3.60 and 8.00 mg/L test item. Each treatment concentration was prepared separately. The average recoveries of 0.0200 mg/L and 0.160 mg/L Al-spiked samples were 108% and 94.3% with RSDs of 6.46% and 1.29%, and the average recoveries of 0.0200 mg/L and 0.160 mg/L V-spiked samples were 97.3% and 96.8% with RSDs of 0.28% and 1.05%, and the average recoveries of 15 mg/L and 30 mg/L Mg-spiked samples were 106% and 91.9% with RSDs of 5.99% and 5.02%.

The 21 days NOELR was determined to be 0.160 mg/L (corresponding to the time-weighted mean measured concentrations of Al, V and Mg of 0.00833, 0.00213 and 8.64 mg/L). The 21 days LOELR value was determined to be 0.340 mg/L (corresponding to the time-weighted mean measured concentrations of Al, V and Mg of 0.00821, 0.00320 and 8.48 mg/L). The 21 days EL50 value was 3.71 mg/L (95% confidence limits: 1.83-54.4 mg/L).