reaction mass of 1-[2-(2-aminobutoxy)ethoxy]but-2-ylamine and 1-({[2-(2-aminobutoxy)ethoxy]methyl}propoxy)but-2-ylamine

Toxicity to terrestrial plants

Administrative data

Endpoint:

toxicity to terrestrial plants: long-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2011-03-24 - 2011-07-25

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)

Deviations:

yes

Remarks:

: not affecting the outcome of the test

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Test material form:

liquid

Specific details on test material used for the study:

- Name of test material (as cited in study report): XTJ 568
- Physical state: liquid (extremely pale yellow)
- Analytical purity: 97.2%
- Lot/batch No.: DR32630507
- Expiration date of the lot/batch: 31 December 2011
- Storage condition of test material: at room temperature in the dark

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Duplicate samples were taken from deionized reverse osmosis water and the 10 g/L stock solution.
- Samples were stored at ca. -20°C for further analysis if necessary.

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

- Method of mixing into soil (if used): addition of stock solution (in deionized reverse osmosis water) to soil and mixing with a Hobart A200N mixer
- Controls: idem, but addition of a similar amount of deionized reverse osmosis water without added test substance

Test organisms

1

Species:

Avena sativa

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

- Common name: oat
- Plant family: Poaceae
- Variety: SW Argyle
- Source of seed: Senova Ltd., Cambridge, UK
- Prior seed treatment/sterilization: none
- Seed storage: cool, dry conditions
- Percentage germination: 98% (data supplied by supplier)

2

Species:

Lycopersicon esculentum

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: tomato
- Plant family: Solanaceae
- Variety: Orange Pixie
- Source of seed: Herbiseed, Twyford, UK
- Prior seed treatment/sterilization: none
- Seed storage: cool, dry conditions
- Percentage germination: 82% (data supplied by supplier)

3

Species:

Glycine max (G. soja)

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: soybean
- Plant family: Fabaceae
- Variety: Amsoy
- Source of seed: Herbiseed, Twyford, UK
- Prior seed treatment/sterilization: none
- Seed storage: cool, dry conditions
- Percentage germination: 86% (data supplied by supplier)

Study design

Test type:

seedling emergence toxicity / vegetative vigour test

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

21 d

Remarks:

i.e., 21 days after 50% emergence in the control

Test conditions

Test temperature:

10-42°C - study plan: 10-35°C. Temperatures outside the study plan range were considered not to affect the integrity of the outcome of the study as no morphological abnormalities were observed throughout the test.

pH:

not reported

Moisture:

not reported

Details on test conditions:

TEST SYSTEM
- Testing facility: greenhouse with artificial lighting and heating systems
- Test container (type, material, size): polypropylene pots, depth 9.9 cm, diameter 13 cm, placed in plant pot saucer serving as sub-irrigation reservoir
- Amount of soil: ca. 850 g (dw)
- Method of seeding: planting depth according to recommendations of supplier, initial surface irrigation following sowing to encourage germination
- No. of seeds per container: 5
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Artificial sandy loam soil mixed with sand (50/50 w/w) to give a nominal organic carbon content of 0.87% (eq. to 1.5% organic matter, which is within the acceptable range for substrates to be used in plant toxicity tests)
- Supplier: Sporting Surface Supplies Ltd., Reigate, Surrey, UK
- The soil was characterised by NRM Laboratories
- Organic carbon (%): 0.87%
- Dry matter: 88.4% w/w
- Nitrate Nitrogen: 44.74 mg/kg
- Ammonium Nitrogen: 10.08 mg/kg
- Total Nitrogen: 0.14 % w/w
- Total Phosphorus: 461 mg/kg
- Total Potassium: 1010 mg/kg
- pH: 8.0
- Organic matter: 3.8%
- Sand (2.00 - 0.063 mm): 90 % w/w
- Silt (0.063 - 0.002 mm): 5% w/w
- Clay (<0.002 mm): 5 % w/w
- Available Nitrogen: 219.3 kgN/ha
- Available Phosphorus: 72.8 mg/L
- Available Potassium: 991 mg/L
- Available Magnesium: 144 mg/L
- Conductivity: 3009 µS/cm

GROWTH CONDITIONS
- Photoperiod: 16 h light, 8 h dark
- Light source: artificial, triphosphor fluorescent lamps
- Light intensity and quality: 58 wtt, 240 volt triphosphor fluorescent lamps at 5000 lumen
- Day/night temperatures: no details reported
- Relative humidity (%): 16-87%
- Watering regime and schedules: no details reported (top-watering only to stimulate germination, afterwards only sub-irrigation)
- Water source/type: no details reported
- Volume applied: no details reported

EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Emergence: daily after sowing
- Mortality and/or morphological abnormalities: daily for 21 days following 50% emergence in control
- Shoot dry weight: day 21 (harvested, dried at 60°C)

TEST CONCENTRATIONS
- Limit test (1000 mg/kg)
- Range finding study: yes
- Test concentrations in range finding study: control + 10, 100 and 1000 mg/kg
- Results used to determine the conditions for the definitive study: yes (except the results for soybean due to weak emergence)

Nominal and measured concentrations:

Definitive study (limit test): control + 1000 mg/kg

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Species:

Lycopersicon esculentum

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg soil ww

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

and emergence

Effect concentrations 2

Species:

Lycopersicon esculentum

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/kg soil ww

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

and emergence

Effect concentrations 3

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg soil ww

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

and emergence

Effect concentrations 4

Species:

Glycine max (G. soja)

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/kg soil ww

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

and emergence

Effect concentrations 5

Species:

Avena sativa

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/kg soil ww

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

and emergence

Effect concentrations 6

Species:

Avena sativa

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/kg soil ww

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks:

and emergence

Details on results:

No morphological abnormalities were observed for any of the species throughout the test.

Reported statistics and error estimates:

Statistical analysis of emergence and dry weight data was performed using Bartlett's test for homogeneity of variance (Sokal and Rohlf, 1981) and a Students t-test. For comparison of several treatments with the control, Dunnett's multiple comparison procedure was used.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In this seedling emergence and growth test, the effect of the test substance on emergence and growth (shoot dry weight) was investigated up to 21 days after 50% emergence in the control was reached. Three plant species were included in the study: tomato (Lycopersicon esculentum), soybean (Glycine max), and oat (Avena sativa). Based on the results of the range finding test, the definitive test was decided to be a limit test (control + 1000 mg/kg). At the single concentration of 1000 mg/kg (ww), no statistically significant effects were observed on any of the species. Therefore, all EC50 and NOEC values were > 1000 mg/kg (ww) and >= 1000 mg/kg (ww), respectively.