Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 447-920-2 | CAS number: 897393-42-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): XTJ 568
- Physical state: Clear colourless liquid
- Analytical purity: 97.8%
- Lot/batch No.: 8191-34
- Expiration date of the lot/batch: 01 January 2004
- Storage condition of test material: at room temperature in the dark
- Other: specific gravity: > 0.94 g/cm3 - Analytical monitoring:
- yes
- Details on sampling:
- Concentrations:
- Range-finding test: 10 mg/L
- Final test: at the start of testing (control, 2.2, 22, and 100 mg/L) and at the end of testing (all test concentrations)
Sampling: duplicate 10-mL samples from each sampled concentration
Sampling frequency:
- Range-finding test: t=0, t=24, t=72
- Final test: see above
Sampling storage: not applicable, immediate analysis - Vehicle:
- no
- Details on test solutions:
- - Preparation of test solutions started with a stock solution of 100 mg/L in test medium, applying a 15-minute stirring period.
- Preparation of lower test concentrations through subsequent dilutions of the stock in test medium.
- Since pH of stock solution was quite high (alkaline) (outside optimum range for testing with algae (6.0-9.0)), pH was adjusted to 8.1 with 1 N HCl in the final test to exclude possible pH related effects. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Strain: NIVA CHL 1
- Source (laboratory, culture collection): in-house laboratory culture
- Age of inoculum (at test initiation): 3-4 days (from pre-culture)
- Method of cultivation: stock cultures started by inoculating M1 growth medium with algal cells from a pure culture on agar; suspensions continuously aerated, kept at 23±2°C, and continuous illumination (60-120 µE2/m2/s, wavelength 400-700 nm)
ACCLIMATION
- Pre-culture in M2 (ISO) medium during 3 to 4 days prior to testing
- Pre-culture medium is same as test medium
- Pre-culture kept at same conditions as test conditions
- Initial density of pre-culture: 2E04 cells/mL. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- 24 mg CaCO3/L
- Test temperature:
- 23.5-24°C (measured in temperature control vessel)
- pH:
- 8.0-10.0 (measured at the beginning and at the end of the test).
pH of the control, 2.2 and 10 mg/L test groups was > 9.0 and in addition pH of the 2.2 mg/L test group increased > 1.5 unit during the test period. Since this was related to a relatively high rate of algal growth this deviation was not considered to have affected the validity of the test. - Dissolved oxygen:
- Not applicable
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Range-finding test:
- Nominal concentrations: Control + 0.1-1.0-10-100 mg/L
- Measured concentrations (only 10 mg/L): 71-104% of nominal
Final test:
- Nominal concentrations: Control + 2.2-4.6-10-22-46-100 mg/L
- Measured concentrations at start of testing: 96-88% of nominal for 22 and 100 mg/L and 44% of nominal for 2.2 mg/L
- Measured concentrations at end of testing: 98-107% of nominal for 4.6 up to 100 mg/L and 47-51% of nominal for 2.2 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL, all-glass, containing 50 mL of test medium
- Aeration: no, but continuous shaking
- Static system
- Initial cells density: 1E04 cells/mL
- Control end cells density: in the controls, cell density increased by an average factor of > 16 within 3 days (106.91-143.68 x 10^4 cells/mL)
- Replicates: 3 replicates of each test concentration; 6 replicates of the blank-control; 1 replicate of the highest concentration without algae
GROWTH MEDIUM
- Standard medium used in test and pre-culture: yes, M2 (ISO)
- Standard medium used for culturing: yes, M1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Milli-Q (tap water purified by reverse osmosis (milli-RO) and passed over activated carbon and ion-exchange cartridges)
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: pH of test media adjusted to 8.1 with 1 N HCl prior to testing, no adjustment during the test
- Photoperiod: continuous illumination
- Light intensity and quality: 80 to 115 µE/m2/s (TLD-lamps of the type Cool-white of 30 Watt)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Cell density was determined at 24, 48, and 72 h
- At the beginning of the test, cells were counted by microscope, using a counter chamber.
- Thereafter, cell densities were determined by spectrophotometric measurement of samples at 720 nm using a Varian Cary 50 single beam spectrophotometer with immersion probe (pathlength = 20 mm), Varian Nederland BV., Houten, The Netherlands. Algal medium was used as blank.
- Quantification of cell densities was based on a calibration curve.
TEST CONCENTRATIONS
- Range-finding test: Control + 0.1-1.0-10-100 mg/L
- Final test: Control + 2.2-4.6-10-22-46-100 mg/L
- Results used to determine the conditions for the definitive study: the range-finding study showed that the expected EC50 for cell growth inhibition was between nominal concentrations of 10 and 100 mg/L and for growth rate reduction was > 100 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI = 7.3-52 mg/L
- Details on results:
- Growth rate:
72-h NOEC, LOEC, EC10 and EC50 = 4.6, 10, 20, and > 100 mg/L, respectively.
Biomass:
72-h NOEC, LOEC, EC10 and EC50 = 4.6, 10, 4.4, and 44 mg/L, respectively. - Results with reference substance (positive control):
- 72-h EbC50 = 0.59 mg/L (95% CI = 0.38-0.92 mg/L), i.e., within ISO range of 0.20 to 0.75 mg/L
72-h ErC50 = 0.89 mg/L (95% CI = 0.56-1.4 mg/L), i.e., within ISO range of 0.60-1.03 mg/L - Reported statistics and error estimates:
- NOEC determined using ANOVA, Tukey test, TOXSTAT Release 3.5, 1996.
EC50 and EC10 calculated based on linear regression analysis of the percentages of growth rate reduction versus logarithms of corresponding nominal concentrations of test substance. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this study with the unicellular freswhater green alga Selenastrum capricornutum (Pseudokirchneriella subcapitata), XTJ 568 reduced growth rate significantly at nominally 10 mg/L and higher. The 72-h NOEC, LOEC, EC10 and EC50 based on growth rate were 4.6, 10, 20 and > 100 mg/L, respectively. The test results are valid and the study can be considered a Klimisch 1 study.
Reference
Description of key information
The Klimisch 1 study by Bouwman (2004) performed at NOTOX is considered the key study. This algal growth inhibition test with the unicellular green alga Selenastrum capricornutum (Pseudokirchneriella subcapitata), performed according to ISO Guideline 8692, EU Method C.3 and OECD Guideline 201, yielded a 72-h NOEC and EC50 (based on growth rate reduction) of 4.6 and > 100 mg/L, respectively. These values can be used as key values for PNEC derivation.
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 4.6 mg/L
Additional information
A single algal growth inhibition study is available (Bouwman, 2004). Under the conditions of this study with the unicellular freshwater green alga Selenastrum capricornutum (Pseudokirchneriella subcapitata), the substance reduced algal growth rate as well as biomass significantly at nominally 10 mg/L and higher. The 72-h NOEC, LOEC, EC10 and EC50 based on algal biomass were 4.6, 10, 4.4 and 44 mg/L, respectively, whereas the 72-h NOEC, LOEC, EC10 and EC50 based on algal growth rate were 4.6, 10, 20 and > 100 mg/L, respectively. The results of this test are valid and the study is considered a Klimisch 1 study.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
