reaction mass of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-2H -isothiazol-3-one

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Test material form:

other: Amber liquid

Details on test material:

- Name of test material (as cited in study report):Kathon(TM) 886F biocide

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Rohm and Haas, Batch No. 6111L019

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Stability under test conditions: Stable at room temperature
- Solubility and stability of the test substance in the solvent/vehicle: Soluble and stable
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: No

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Dilution in water
- Final dilution of a dissolved solid, stock liquid or gel: 1, 10, 50, 100 and 500 mg/L

OTHER SPECIFICS: Purity of test material was 14.13%

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

- Method of mixing into soil (if used): Laboratory mixer
- Controls: Negative control

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: Earthworm (Eisenia foetida andrei)
- Source: Blades Biological, Kent
- Age at test initiation (mean and range, SD): 2 months
- Weight at test initiation (mean and range, SD): 300-600 mg

ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): acclimated in artificial soil for at least 7 days prior to study initiation
- Health during acclimation (any mortality observed): Not described

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Test conditions

Test temperature:

20 - 21 °C

pH:

6.8-7.0

Moisture:

Approximately 35 %

Details on test conditions:

TEST SYSTEM
- Test container (material, size): Glass beakers of 1 liter capacity covered with perforated plastic film
- Amount of soil or substrate: 750 g
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): 20 % kaolinite clay, 70 % sand, 8 % sphagnum peat moss

OTHER TEST CONDITIONS
- Photoperiod: Not described
- Light intensity: 608 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Worms: daily observations, mortality (days 7 and 14), live weights (days 0 and 14).
Soil: moisture content, pH
Environmental area: temperature

VEHICLE CONTROL PERFORMED: not applicable

Nominal and measured concentrations:

Nominal: 0, 62.5, 125, 250, 500 and 1000 mg whole product /kg dry weight soil

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

8.8 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality

Effect concentrations 2

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

86.6 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality

Effect concentrations 3

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

0.71 mg/kg soil dw

Nominal / measured:

meas. (TWA)

Conc. based on:

act. ingr.

Basis for effect:

mortality

Effect concentrations 4

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

6.96 mg/kg soil dw

Nominal / measured:

meas. (TWA)

Conc. based on:

act. ingr.

Basis for effect:

mortality

Details on results:

- Mortality at end of exposure period: No mortality observed in control, 87.5 % at the highest concentration after 14d. Other treatments showed 17.5% or lower mortality.
- Total mass of adults at beginning of test: Not described
- Changes in body weigth of live adults (% of initial weight) at end of exposure period: Kathon™ 886F had no significant effect on earthworm weight under the conditions of this test.
- No. of offspring produced: Not described
- No. of unhatched cocoons: Not described
- Morphological abnormalities: Not described
- Behavioural abnormalities: No
- Other biological observations: Earthworms at 1000 and 500 mg/kg were observed on the soil surface from test commencement through day 10.

Reported statistics and error estimates:

The median lethal concentration (LC50) at 7 and 14 days was determined by probit analysis (Finney D.J., 1971, Probit analysis, 3rd edition, Cambridge University Press). Differences in body weight were analyzed by analysis of variance (ANOVA). Pairwise comparisons between each of the doses and the control were performed using Dunnett’s test at the 5 % significance level. The probit transformed concentrations of TS were subject to a regression procedure against logarithmically transformed concentrations of TS with a Newton-Raphson maximum likelihood iterative procedure being used to obtain parameter estimates. From the model the LC50 value was estimated. The goodness of fit was checked via the Pearson chi-squared test statistic. A significant chi-squared test statistic indicated heterogeneity of variances between the observed and expected % mortality values. If the test statistic was significant at the 1 % level, the variances and covariances were adjusted by a heterogeneity factor. Following this, the confidence limits were not reported and the LC50 values should be treated with caution.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

Mortality of control animals < 10 %

Conclusions:

14d LC50 (survival, TWA) = 6.96 mg a.i. /kg dry soil ; 14d LC50 (survival, nominal) = 86.6 mg a.i. /kg dry soil
14d NOEC (survival, TWA) = 0.71 mg a.i. /kg dry soil; 14d NOEC (survival, nominal) = 8.83 mg a.i. /kg dry soil

Executive summary:

OECD Method 207, Acute toxicity to the earthworm. Kathon™886F had no significant effect on earthworm weight under the conditions of this test. KathonTM 886F is not highly toxic to earthworms over a 14-day test period. 14 dLC50= 613 mg/kg whole product (soil); 86.6 mg a.i./kg (soil); 14 d NOEC = 62.5 mg whole product/kg (survival); 8.8 mg a.i./kg.