Isobutyl (S)-2-chloropropionate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study according to the German National Standard (DIN).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412 part 15

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Isobutyl-2-chloropropionate

IUPAC Name:

Isobutyl-2-chloropropionate

Constituent 2

Reference substance name:

Isobutyl (S)-2-chloropropionate

EC Number:

280-349-5

EC Name:

Isobutyl (S)-2-chloropropionate

Cas Number:

83261-15-8

Molecular formula:

C7H13ClO2

IUPAC Name:

isobutyl (S)-2-chloropropanoate

Details on test material:

- Name of test material (as cited in study report): L-CHLORPROPIONSAEURE-ISOBUTYLESTER
- Analytical purity: 99.8%

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe (Leuciscus idus)
- Source: Fischzucht Paul Eggers, 2354 Hohenweststedt, Germany
- Length at study initiation (length definition, mean, range and SD): 6.1 cm (range 5.7 - 6.6 cm)
- Weight at study initiation (mean and range, SD): 2.8 g (range 2.4 - 3.9 g)
- Feeding during test: Withdrawal of food 1 day before and during exposure


ACCLIMATION
- Acclimation period: about 11 weeks
- Health during acclimation (any mortality observed): 0%

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96

Remarks on exposure duration:

h

Test conditions

Hardness:

about 2.5 mmol/l

Test temperature:

21 °C

pH:

7.7 - 8.2

Dissolved oxygen:

7.7 - 9.0 mg/l

Nominal and measured concentrations:

Nominal: 2.15, 4.64, 10.0, 21.5, 46.4 and 100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: All glass aquaria (30 x 22 x 24 cm), Volume of water: 10 L
- Aeration: Continuous aeration with oil-free air, slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 2.8 g fish/L test water


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, Part II, draft 1981
- Alkalinity: pH about 8.0
- Ca/mg ratio: 4 : 1


OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime


EFFECT PARAMETERS MEASURED:
observations: mortality and symptoms after 0, 1, 24, 48, 72, and 96 h
measurements: pH and oxygen content after 0, 24, 48, 72 and 96 h


TEST CONCENTRATIONS
Nominal test concentrations: 2.15, 4.64, 10.0, 21.5, 46.5 and 100 mg/l. In addition, a control was tested in parallel.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)