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EC number: 206-058-5 | CAS number: 298-12-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to reproduction: other studies
Administrative data
- Endpoint:
- toxicity to reproduction: other studies
- Type of information:
- other: Literature data
- Adequacy of study:
- other information
- Study period:
- 2001
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Bibliographical data.
Data source
Reference
- Reference Type:
- publication
- Title:
- Effects of ethylene glycol and metabolites on in vitro development of rat embryos during organogenesis
- Author:
- Klug S., Merker H.J., Jäckh R.
- Year:
- 2 001
- Bibliographic source:
- Toxicology in vitro, 15, 635-642.
Materials and methods
- Principles of method if other than guideline:
- The aim of this study was to investigate in vitro the relative impact of ethylene glycol, a major industrial chemical, and its individual metabolites on the embryonic development of rats.
Rat whole embryos were exposed for 48 h (Day 9.5-11.5 of gestation) to ethylene glycol (EG), and its metabolites glycolaldehyde (GAI), glycolic acid (GA), glyoxylic acid (GXA), glyoxal (GXAI) and oxalic acid (OXA) at increasing concentrations. - GLP compliance:
- not specified
- Type of method:
- in vitro
Test material
- Reference substance name:
- Automatically generated during migration to IUCLID 6, no data available
- IUPAC Name:
- Automatically generated during migration to IUCLID 6, no data available
- Details on test material:
- Aqueous concentration not precised
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Details on test animals or test system and environmental conditions:
- Wistar rats (Bor: Wisw/SPF, TNO) were kept under SPF conditions under a constant day/night cycle from 9.00 to 21.00 at 21 +/- 1°C and 50 +/- relative humidity. The animals received pellet feed and tap water ad lib.
Three females were caged together with one male from 6.00 to 8.00 am; if sperm was detected in the vaginal smear, the following 24 h were determined as day 0 of pregnancy.
Pregnant Wistar rats were killed on gestation day 9.5.
Head-fold-stage conceptuses were removed under aseptic conditions from the uterine wall and transferred to a petri dish filled with preparation medium
Administration / exposure
- Route of administration:
- other:
- Duration of treatment / exposure:
- 48 h
- Details on study design:
- Wistar rats (Bor: Wisw/SPF, TNO) were kept under SPF conditions under a constant day/night cycle from 9.00 to 21.00 at 21 +/- 1°C and 50 +/- relative humidity. The animals received pellet feed and tap water ad lib.
three females were caged together with one male from 6.00 to 8.00 am; if sperm was detected in the vaginal smear, the following 24 h were determined as day 0 of pregnancy.
Pregnant Wistar rats were killed on gestation day 9.5. Head-fold-stage conceptuses were removed under aseptic conditions from the uterine wall and transferred to a petri dish filled with preparation medium.
Embryos were cultured for 48 h in suplemented bovine serum as culture medium at 38.5°C in a roller device (25 rpm).
The serum was delayed centrifuged (30 min), heat inactivated, sterile filtered and supplemented with Tyrode's buffer (6:1) and enriched with methionine (75 µg/ml). The culture bottles were initially gassed with 10 vol% O2, 5 vol% CO2 and 85 vol% N2. After 36 h, the O2 concentration was increased to 50 vol%. All substances were tested twice after dissolution in bi-distilled water, in at least two concentrations in two independent experimental series.
The studied concentrations were as follows:
Ethylene glycol: 50, 100 and 200 mM,
Glyoxal: 3 and 6 mM,
Glycolic acid: 1, 3, 6 and 10 mM,
Oxalic acid: 1 and 2 mM,
Glyoxylic acid: 0.3 and 1 mM,
Glycolaldehyde: 0.03, 0.1, 0.2 and 0.3 mM.
Results and discussion
Any other information on results incl. tables
Glyoxylic
acid (GXA) revealed no detectable effects at a 0.3 mM concentration in
the culture medium. The slight but significant reduction of the yolk sac
diameter is considered to be biologically insignificant since the growth
parameters crown rump and protein content were not affected.
At 1 mM, all values for growth and differentiation were severely
affected and a 100 % rate of dysmorphogenesis embryos (concerning the
shape) was recorded. 13 embryos showed a retardation of the head anlage
and six embryos an open ear vesicle. In nine embryos, accurate counting
of the somite anlage was not possible.
The pattern of dysmorphogenesis with all compounds including EG showed a
general embryotoxicity with diffusely distributed cell necrosis with no
specific target tissues selectively affected.
Embryotoxic concentrations were achieved within the following range:
Ethylene glycol: 100 and 200 mM,
Glycolic acid: 3 mM,
Glyoxal: 6 mM,
Oxalic acid: 1 and 3 mM,
Glyoxylic acid: 0.3 and 1 mM,
Glycolaldehyde: 0.1 and 0.2 mM.
Applicant's summary and conclusion
- Conclusions:
- The results obtained in this study emphasize the hypothesis that the metabolites and not ethylene glycol itself are responsible for the embryotoxicity of ethylene glycol in rats. And in conclusion the Authors hypothesized that Glycolic acid concentrations in plasma and extra-embryonic fluids are the predominant factor for ethylene glycol developmental effects in rats.
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