Glyoxylic acid

Additional toxicological data

Administrative data

Endpoint:

additional toxicological information

Type of information:

other: Literature data

Adequacy of study:

other information

Study period:

1993

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Literature data

Data source

Reference

Reference Type:

publication

Title:

The absence of glyoxylate cycle enzymes in rodent and embryonic chick liver

Author:

Holmes RP

Year:

1993

Bibliographic source:

Biochimica et Biophysica Acta, 1158, 47-51.

Materials and methods

Type of study / information:

Absence of glyoxylate cycle enzymes in rodent and embryonic chick liver.

Principles of method if other than guideline:

The aim of this study was to determine the absence of glyoxylate cycle enzymes in rodent and embryonic chick liver.
Reaction products in these assays have been measured principally by chromatographic separation of isotopes or by colorimetric procedures.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Glyoxylic acid

EC Number:

206-058-5

EC Name:

Glyoxylic acid

Cas Number:

298-12-4

Molecular formula:

C2H2O3

IUPAC Name:

2-oxoacetic acid

Results and discussion

Any other information on results incl. tables

Reversed phase HPLC revealed the absence of detectable isocitrate lyase activity in guinea pig, rat and chick embryonic liver. The formation of several other alpha-keto acids was detected and this may account for the previously reported activities.
Using HPLC to monitor malate formation malate synthase activity was not detected in these tissues.

Applicant's summary and conclusion

Conclusions:

These results indicate that when assaying enzyme activities in crude tissue homogenates specific methods for the identification of end products are required.