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EC number: 246-278-9 | CAS number: 24468-13-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-ethylhexyl chloroformate
- EC Number:
- 246-278-9
- EC Name:
- 2-ethylhexyl chloroformate
- Cas Number:
- 24468-13-1
- Molecular formula:
- C9H17ClO2
- IUPAC Name:
- 2-ethylhexyl carbonochloridate
- Details on test material:
- - Name of test material (as cited in study report): 2-Ethyihexylchiorformiat
- internal Test substance No .: 99/34-1
- Lot/batch No.: Vers. Nr. 175/Y
- Date of manufacture : January 14, 1999
- Analytical purity: 99,5% (GC)
- Appearance, consistency: Colorless, liquid
- Storage: Room temperature
Constituent 1
Method
- Target gene:
- Salmonella Typhimurium: (his-)
E. Coli: (Trp-)
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- Standard S9-Mix
- Test concentrations with justification for top dose:
- Experiment 1: 0; 20; 100 ; 500 ; 2,500 and 5,000 µg/plate
experiment 2&3: 0; 0 .8 ; 4; 20; 100 and 500 pg/plate - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: soulability
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene (2-AA)
- Remarks:
- all strains with S9 mix
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: N-methyl-N'-nitro-N-nitrosoguanidine ( MNNG)
- Remarks:
- TA1535; TA 100 without S9 mix
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 4-nitro-o-phenylendiamine (NOPD)
- Remarks:
- TA 98 without S9 mix
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- TA 1537 without S9 mix
- Positive control substance:
- 4-nitroquinoline-N-oxide
- Remarks:
- E . coli WP2 uvrA without S9 mix
- Details on test system and experimental conditions:
- METHOD OF APPLICATION
Standard plate test and preincubation test
DURATION
- Preincubation period: 20 min
- Expression time : 48 - 72 hours
SELECTION AGENT (mutation assays):
The Salmonella strains are checked for the following characteristics at regular intervals: deep rough character (rfa) ; UV sensitivity (o uvrB); ampicillin resistance (R factor plasmid). E . coli WP2 uvrA is checked for UV sensitivity. Histidine and tryptophan auxotrophy is automatically checked in each experiment via the spontaneous rate.
NUMBER OF REPLICATIONS: 3
DETERMINATION OF CYTOTOXICITY
Backgound
- Evaluation criteria:
- Positive results
- doubling of the spontaneous mutation rate (control)
- dose-response relationship
- reproducibility of the result - Statistics:
- mean and SD of replicates
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Effects of pH: not reported
- Effects of osmolality: not reported
- Evaporation from medium: not expected (vapour pressure 0.4 mbar)
- Water solubility: not reported
- Precipitation: No
- Other confounding effects: None
RANGE-FINDING/SCREENING STUDIES:
Experiment1 was the screening study
COMPARISON WITH HISTORICAL CONTROL DATA:
The number of revertants for all tester strains were within the historical negative control range under all experimental conditions in two experiments carried out independently of each other.
ADDITIONAL INFORMATION ON CYTOTOXICITY:
A bacteriotoxic effect (reduced his or trp" background growth, decrease in the number of his+ or trp+ revertants, reduction in the titer) was observed depending on the strain and test conditions from about 100 pg - 500 pg/plate onward . - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Toxicity was not observed in cells treated with > = 2500 micrograms/plate (standard plate test) and >= 100 micrograms/plate (preincubation test). The test material did not precipitate. Positive controls induced at least a 6-fold increase in mutants.
Salmonella:
Standard plate test: There was no mutagenic effect of test material in the presence or absence of S-9. In the absence of S-9, the average numbers of mutants in controls experiments in strains TA98, TA100, TA1535 and TA1537 in both experiments were 23 and 27, 127 and 142, 19 and 21, and 10 and 12, respectively. The average numbers of mutants in treated strains TA98, TA100, TA1535 and TA1537 in both experiments ranged from 2-24,2-145, 5-22 and 1-9, respectively. In the presence of S-9, the average numbers of mutants in controls in strains TA98, TA100, TA1535 and TA1537 in both experiments were 34 and 36, 133 and 144, 19 and 20 and 10 and 11, respectively. The average numbers of mutants in treated strains TA98, TA100, TA1535 and TA1537 in both experiments with S-9 ranged from 1-30, 3-156, 1-20 and 1-13, respectively.
Preincubation
test: There was no mutagenic effect of test material in the presence or
absence of S-9. In the absence of S-9, the average numbers of mutants in
controls in strains TA98, TA100, TA1535 and TA1537 were 26, 122, 19 and
11, respectively. The average numbers of mutants in treated strains
TA98, TA100, TA1535 and TA1537 ranged from 19-30, 118-127, 10-15 and
6-10, respectively. In the presence of S-9, the average numbers of
mutants in controls in strains TA98, TA100, TA1535 and TA1537 were 32,
119, 19 and 10, respectively. The average numbers of mutants in treated
strains TA98, TA100, TA1535 and TA1537 with S-9 ranged from 26-33,
104-132, 14-17 and 6-14, respectively.
E.Coli.
Standard plate test: There was no mutagenic effect of test material in the presence or absence of S-9. In the absence of S-9, the average numbers of mutants in controls in the 2 experiments were 23 and 29. The averagenumber of mutants in treated cells in the 2 experiments ranged from 14-27. In the presence of S-9, the average numbers of mutants in controls in the 2 experiments were 34 and 35. The average number of mutants in treated cells (both experiments) ranged from 14-36.
Preincubation test: There was no mutagenic effect of test material in the presence or absence of S-9. In the absence of S-9, the average number of mutants in the control was 28. The average number of mutants in treated cells ranged from 32-35. In the presence of S-9, the average number of mutants in the control was 25. The average number of mutants intreated cells ranged from 18-28.
Applicant's summary and conclusion
- Conclusions:
- The test substance 2-Ethylhexylchlorformiat is not mutagenic in the Salmonella typhimurium/Escherichia coli reverse mutation assay under the experimental conditions chosen here.
- Executive summary:
2-Ethyihexylchiorformiat was tested for its mutagenic in an Ames Test. The test strains waer TA1535, TA 100, TA 1537, Ta 98 and E.coli WP2 uvrA in a dose range of 0.8 µg - 5000 µg/plate (SPT) and 0.8 µg - 500 µg/plate (PIT). Standard plate test (SPT) and preincubation test (PIT) both with and without metabolic activation (Aroclor-induced rat liver S-9 mix). No precipitation of the test substance was found. A bacteriotoxic effect was observed depending on the strain and test conditions from about 100 µg - 500 µg/plate onward. An increase in the number of his+ or trp+ revertants was not observed in the standard plate test or in the preincubation test either without S-9 mix or after the addition of a metabolizing system. According to the results of the present study, the test substance 2-Ethylhexylchlorformiat is not mutagenic in the Salmonella typhimurium/Escherichia coli reverse mutation assay under the experimental conditions chosen here .
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