N-(carboxymethyl)-N-(phosphonomethyl)glycine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2005.05.18-2005.05.20

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DS/EN ISO 14669

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

N-(carboxymethyl)-N-(phosphonomethyl)glycine

EC Number:

227-824-5

EC Name:

N-(carboxymethyl)-N-(phosphonomethyl)glycine

Cas Number:

5994-61-6

Molecular formula:

C5H10NO7P

IUPAC Name:

2-[(carboxymethyl)(phosphonomethyl)amino]acetic acid

Details on test material:

- Name of test material (as cited in study report): PMIE or Phosphonomethyliminodiacetic acid

- Substance type: Solid
- Physical state: White powder
- Analytical purity: 99 % w/w

- Purity test date: 15.06.2005
- Lot/batch No.: SB-7-4-89
- Expiration date of the lot/batch: 11.09.2005
- Stability under test conditions: Stable for 6 months
- Storage condition of test material: at - 20 ˚C

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 2000 mg/L stock solution of 400 mg PMIE in 200 mL seawater (32 ‰), diluted with seawater, was analysed
- Sample storage conditions before analysis: at minus 20 ˚C

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Stock solution: Nominiel concentration 2000 mg/l, actual concentration 1990 mg/l
- Evidence of undissolved material (e.g. precipitate, surface film, etc): None

Test organisms

Test organisms (species):

other aquatic crustacea: Acartia tonsa

Details on test organisms:

TEST ORGANISM
- Common name: Marine copepod
- Strain: DHI Water and Invironment (the former VKI) 1987
- Source: DHI Water and Invironment (the former VKI)
- Age at study initiation (range): 2-3 weeks


ACCLIMATION
- Acclimation conditions : no acclimatation needed, test done under same conditions at same facility as test animals was optained from

Study design

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

19.5 ±0.5 ˚C

pH:

7.9-8.2

Dissolved oxygen:

>97%

Salinity:

32 ‰

Nominal and measured concentrations:

Stock solution: Nominal: 2000 mg/L, measured:1990 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 ml glass beaker
- Type : open
- Material, size, headspace, fill volume: glass. 50 ml, 10cm, 25 ml
- Aeration: yes
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 5

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater from the North Sea, filtered through Millipore filters (10; 5; 0.5; 0.22 μm)
- Particulate matter: None
- Culture medium different from test medium: No


OTHER TEST CONDITIONS
- Adjustment of pH: None
- Photoperiod: light/dark 16:8 hours

TEST CONCENTRATIONS

- Test concentrations: 0 (control);100; 200; 500; 1000 and 2000 mg/l
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol, CAS No. : 591-35-5, at 0.1; 0.18; 0.32; 0.56; 1.0; 1.8 and 3.2 mg/L.

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 2 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Results with reference substance (positive control):

- Results with reference substance valid?: Yes
- EC50/LC50: 1.39 mg/l
- Other: Result within normal acceptable range, 0.5-1.5 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

LC50(48 h) of N-(carboxymethyl)-N-(phosphonomethyl)-glycine, CAS No. 5994-61-6, exceeds 2000 mg/L for Acartia tonsa