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EC number: 217-915-8 | CAS number: 2008-39-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Basic toxicokinetics
Administrative data
- Endpoint:
- basic toxicokinetics
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions: only one dose level was used; no raw data provided (only charts)
Data source
Reference
- Reference Type:
- publication
- Title:
- Oral and dermal application of 2,4-dichlorophenoxyacetic acid sodium and dimethylamine salts to male rats: investigations on absorption and excretion as well as induction of hepatic mixed-function oxidase activities
- Author:
- Knopp, D. and Schiller, F.
- Year:
- 1 992
- Bibliographic source:
- Arch Toxicol, 1992, 66, 170-174
Materials and methods
- Objective of study:
- absorption
- excretion
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 417 (Toxicokinetics)
- Deviations:
- yes
- Remarks:
- only one dose level was used; no raw data provided (only charts)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Dimethylammonium 2,4-dichlorophenoxyacetate
- EC Number:
- 217-915-8
- EC Name:
- Dimethylammonium 2,4-dichlorophenoxyacetate
- Cas Number:
- 2008-39-1
- Molecular formula:
- C8H6Cl2O3.C2H7N
- IUPAC Name:
- dimethylammonium 2,4-dichlorophenoxyacetate
- Details on test material:
- - Name of test material (as cited in study report): Spritz-Hormin 600; 2,4-D dimethylamine salt (DMA)
- Physical state: aqueous concentrate
- Analytical purity: 50%
- Source of the substance: "Chemisches Kombinat”, Bitterfeld, GDR
- Specific activity (if radiolabelling): not stated
Constituent 1
- Radiolabelling:
- yes
Test animals
- Species:
- rat
- Strain:
- other: F1-hybrid of the inbred strains WELS/FOHM and BD IX/Halle
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 320 ± 30 g
- Individual metabolism cages: yes (made of glass, Altromin)
- Diet: Kaninchen-Versuchsfutter ad libitum (Getreidewirtschaft Bernau, Germany)
- Water: tap water ad libitum
- Acclimation period: 1 day
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2
- Humidity (%): 40 - 60
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on exposure:
- PREPARATION OF DOSING SOLUTIONS:
6 mL of Spritz-Hormin 600 (analytical purity 50%) in 1 L water
VEHICLE
- Concentration in vehicle: 3.00 mg/mL
- Amount of vehicle (if gavage): 200 µl - Duration and frequency of treatment / exposure:
- single administration
Doses / concentrations
- Remarks:
- Doses / Concentrations:
1.9 mg/kg body weight
- No. of animals per sex per dose / concentration:
- 5
- Control animals:
- yes, concurrent vehicle
- Details on study design:
- - Dose selection rationale: Dose is representative of occupational exposure
- Details on dosing and sampling:
- PHARMACOKINETIC STUDY (Absorption, distribution, excretion)
- Tissues and body fluids sampled: Urine samples were collected in glass vessels at the urine funnel spout of each metabolism cage.
- Time and frequency of sampling: Urine was collected twice daily at specified tim pointss over a 69 h period. The concentration of 2,4-D was determined immediately.
- other: At the end of the study each animal was sacrificed and blood was taken from the abdominal aorta into heparinized tubes. Blood plasma was obtained by centrifugation. 2,4-D determination was performed by radioimmunoassay (Knopp et al. 1985). If necessary, urine and plasma were diluted with water in order to reach the optimal measuring range of the radioimmunological method. Standard stock solutions of 2,4-D were prepared in methanol. Appropriate dilutions were added to urine or plasma of untreated control rats. Recovery of 2,4-D from fortified samples was 95 - 104%. All samples were assayed in triplicate in a scintillation counter and the data calculated as outlined by Rodbard using a radioimmunoassay program.
Results and discussion
Main ADME resultsopen allclose all
- Type:
- absorption
- Results:
- Cumulative 2,4-D urinary excretion reached about 92% of the applied dose. Assuming an additional 3-4% fecal elimination, systemic availability of the oral dose was nearly 100%.
- Type:
- excretion
- Results:
- One day after dosing approx. 90% of DMA was excreted via urine. Three days after exposure, urinary 2,4-D content could no longer be detected in 50% of the animals.
Toxicokinetic / pharmacokinetic studies
- Details on excretion:
- The highest concentrations of DMA were always measured in the 4.5 h-samples. Peak concentrations were followed by a gradual decline over the next 10 hours. In some animals the concentration reached the "Zero"-level (concentration below the detection limit of 1 µg/L of the analytical method) approx. three days after administration.
Toxicokinetic parametersopen allclose all
- Test no.:
- #1
- Toxicokinetic parameters:
- Tmax: 4.5 h
- Test no.:
- #1
- Toxicokinetic parameters:
- half-life 1st: >20.5 h
Metabolite characterisation studies
- Metabolites identified:
- no
Any other information on results incl. tables
After oral application the volume of urine was significantly increased when compared with the control animals (p< 0.01).
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): no bioaccumulation potential based on study results
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