Iron(II) fumarate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

Experimental data of read across chemicals

Justification for type of information:

Data is from handbook or collection of data

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

WoE was prepared to determine the effect of test chemical on the mortality of fishes.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

other: WoE 2: Danio rerio (previous name: Brachydanio rerio), WoE 3: Lepomis microlophus

Details on test organisms:

TEST ORGANISM
WoE 2:
- Common name:Zebrafish

WoE 3:
- Common name:Bluegill
- Age at study initiation (mean and range, SD):Fingerling
- Length at study initiation (length definition, mean, range and SD):38-76 MM

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Remarks on exposure duration:

96 hours in WoE 3

Test temperature:

WoE 3: 19.5 to 20.5 °C

Dissolved oxygen:

WoE 3: > 60 %

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

245 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: WoE 2

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

300 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: WoE 3

Validity criteria fulfilled:

not specified

Conclusions:

Based on the mortality of fishes Brachydanio rerio (Zebrafish) and Lepomis microlophus (Bluegill) due to the test chemical exposure for 48 hours and 96 hours, the lethal concentration i.e. LC50 value was determined to be in the range of 245 mg/l to > 300 mg/l.

Executive summary:

Summarized result from the various experimental sources for the determination of effect of test chemical on the mortality of fishes are as mentioned below:

An acute toxicity of test chemical was determined on fish. Test conducted under the static system. Brachydanio rerio (Zebrafish) was used as a test organism. Exposure period of 48 hrs was provided. Mortality were used a key parameter for the effect’s measurement. After the exposure period of 48 hours, mortality was calculated and the median lethal concentration (LC50) value was observed to be at 245 mg/L.

Above data further supported by the study from secondary sources. Principle of this study was to determine the effect of test chemical on the mortality of fishes. Lepomis microlophus (Bluegill) was used as a test organism. Test conducted under the static system for 96 hours. Fingerling age with 38-76 MM length fishes was selected for the study. Test temperature 19.5 to 20.5 °C and dissolved oxygen > 60 % were maintained. Based on the mortality of fishes Lepomis microlophus (Bluegill) due to the test chemical exposure for 96 hours, the lethal concentration i.e. LC50 value was determined to be > 300 mg/l. Thus, on the basis of LC50 value, test chemical considers to be nontoxic and not classified as per the CLP classification criteria.

Thus, on the basis of above all studies and effects observations, test chemical consider to be nontoxic and not classified as per the CLP classification criteria.

Description of key information

Based on the mortality of fishes Brachydanio rerio (Zebrafish) and Lepomis microlophus (Bluegill) due to the test chemical exposure for 48 hours and 96 hours, the lethal concentration i.e. LC50 value was determined to be in the range of 245 mg/l to > 300 mg/l.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

245 mg/L

Additional information

Summarized result from the various experimental sources for the determination of effect of test chemical structurally and functionally similar read across chemicals on the mortality of fishes are as mentioned below:

 

An acute toxicity of test chemical was determined on fish. Test conducted under the static system. Brachydanio rerio (Zebrafish) was used as a test organism. Exposure period of 48 hrs was provided. Mortality were used a key parameter for the effect’s measurement. After the exposure period of 48 hours, mortality was calculated, and the median lethal concentration (LC50) value was observed to be at 245 mg/L.

 

Above data further supported by the study from secondary sources. Principle of this study was to determine the effect of test chemical on the mortality of fishes. Lepomis microlophus (Bluegill) was used as a test organism. Test conducted under the static system for 96 hours. Fingerling age with 38-76 MM length fishes was selected for the study. Test temperature 19.5 to 20.5 °C and dissolved oxygen > 60 % were maintained. Based on the mortality of fishes Lepomis microlophus (Bluegill) due to the test chemical exposure for 96 hours, the lethal concentration i.e. LC50 value was determined to be > 300 mg/l. Thus, on the basis of LC50 value, test chemical considers to be nontoxic and not classified as per the CLP classification criteria.

 

Thus, on the basis of above all studies and effects observations, test chemical consider to be nontoxic and not classified as per the CLP classification criteria.