Registration Dossier

Administrative data

Endpoint:
monitoring data
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: No GLP compliance and no data on guidelines.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1980

Materials and methods

GLP compliance:
no
Type of measurement:
natural background concentration
Media:
other: Pseudotsuga menziesii (douglas firs)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Results and discussion

Concentration
Substance or metabolite:
substance
Conc.:
370 mg/kg dw

Any other information on results incl. tables

Effect ofterpenes on the growth of 4 Bacillus species: All the terpenes (including isobornyl acetate, IBA) inhibited Bacillus thuringiensis at levels of the terpene below those found in the foliage of the tree.

Applicant's summary and conclusion

Conclusions:
Concentration of test substance in Douglas fir = 370 mg/kg d.w.
The test substance was inhibitory to the growth of Bacillus thuringiensis.
Executive summary:

These studies report the relation-ship between the Douglas fir terpenes and the growth rate, cellular integrity, mitochondrial activity and infectivity of some selected microorganisms.

The Douglas fir terpene alfa-pinene was shown to inhibit the growth of a variety of bacteria and a yeast. Other terpenes of the Douglas fir, including limonene, camphene, and isobornyl acetate,were also inhibitory to Bacillus thuringiensis. All terpenes were inhibitory at concentrations normally present in the fir needle diet of Douglas fir tussock moth larvae. The presence of such terpenes in the diet of these insects was found to strongly influence the infectivity of B. thuringiensis spores for the Douglas fir tussock moth larvae. The terpene alfa-pinene destroyed the cellular integrity and modified mitochondrial activity in certain microorganisms. The higher concentration of alfa-pinene used in the experiment was assumed to be a reasonable approximation of the effect of total fir needle terpenes on the B. thuringiensis infectivity for Douglas fir tussock moth.