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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
- No chemical analysis. - Stock solution is said to be a dispersion, no information given on homogeneity of the stock or test solutions clearness. As no chemical analyses were performed it is not known if the test concentrations were close to nominal. From the chemical analyses of other studies (Daphnia reproduction, Acute fish etc.) it can be assumed that test concentrations were close to nominal and stable. - No detailed information on test organism (source, culture etc.).
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
see Principles of method if other than guideline
Principles of method if other than guideline:
- No chemical analysis. - Stock solution is said to be a dispersion, no information given on homogeneity of the stock or test solutions clearness. As no chemical analyses were performed it is not known if the test concentrations were close to nominal. From the chemical analyses of other studies (Daphnia reproduction, Acute fish etc.) it can be assumed that test concentrations were close to nominal and stable. - No detailed information on test organism (source, culture etc.).
GLP compliance:
yes
Remarks:
GLP issued by State Environmental Protection Administration (SEPA) of the People’s Republic of China
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
Stock solution of the test substance (1000 mg/L): adding 0.10 g of the test substance to 100 mL of the test water, and then facilitating its dispersion by ultrasonication for 30 min.
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna, not being the first brood progeny, was the test species which were 6 to 24 hours old at the start of the test. Batch No.: DA20120106 and DA20120116.
All organisms used for a particular test originated from cultures established from the same healthy stock of daphnia. The stock animals were maintained in culture conditions (light, temperature, and medium) similar to which used in the test.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Test temperature:
20.1 to 20.8°C
pH:
7.12 to 7.65; without the adjustment of pH during the test
Dissolved oxygen:
86% to 102% of the air saturation
Nominal and measured concentrations:
nominal: 100 and 150 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 100-mL glass beakers
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Light: 16-hour light and 8-hour dark cycle daily
- Feeding: none

TEST MEDIUM:
Good quality tap water which had been dechlorinated for at least 24 h was used. The total hardness of the test water was 122.2 mg (CaCO3)/L, oxygen concentration was about 98% of the air saturation value and pH was 7.55 at room temperature. Characteristics of the dilution water are measured at least twice a year by Jiangsu Provincial Center for Disease Prevention and Control.

RANGE FINDING:
The range-finding test, carried out under static conditions, was regularly conducted to determine the range of concentrations for the following test.
In the range-finding test, groups of animals (5 per group) were exposed to at 3 concentrations of 1, 10 and 100 mg/L (nominal concentration). Synchronously one control group was used in the test. For each test, five daphnia were randomly assigned to 50 mL different concentrations of test substances. During the test no bait was given and no replicates were needed.
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 150 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 150 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
No daphnids died during the test in the control nor in the test concentrations.
Results with reference substance (positive control):
24 h-EC50 was 0.65 mg/L (in the range of 0.6 to 2.1 mg/L).
Validity criteria fulfilled:
no
Remarks:
Chemical analyses were not performed but from other studies (Daphnia reproduction, acute fish etc.) it can be assumed that the test concentrations were stable and close to nominal.
Conclusions:
In combination with the results of the chemical analyses performed in other studies (Daphnia reproduction, acute fish etc.) the result of this study can be considered reliable with restrictions and can be used for risk assessment and classification and labelling.
Executive summary:

According to “The guidelines for the testing of chemicals” (HJ/T 153 -2004) and with reference to Procedure 201of the “Guidelines for Testing of Chemicals” of the OECD: Daphnia sp., Acute Immobilisation Test(2004) etc., this test was designed to determine the acute toxicity of test substance(meta-Diisopropanolbenzene) to Daphnia magna. The study comprised at least one range-finding test followed by a limit test to determine the 48-hour median effective concentration (EC50) of the test substance. In addition, the NOEC (no observed effect concentration) was also determined.

During the whole test period, the pH values of the control mediums and test mediums were between 7.12 and 7.65, and the Dissolved Oxygen (DO) values varied from 86% to 102% of the air saturation. During the test, the temperature of the test mediums were maintained in the range of 20.1oC to 20.8oC, and all daphnia in the control group were normal. With the same conditions, K2Cr2O7 was used as the negative control substance, and the resulting 24 h-EC50was0.65 mg/L. So the study met the acceptability criteria and was considered valid.

Under valid test conditions mentioned above, the 48h EC50is greater than 150 mg/L (nominal concentration). And 48h NOEC value (no observed effect concentration) is greater than 150 mg/L (nominal concentration). According to “The guidelines for the hazard evaluation of new chemical substances (HJ/T 154-2004)”, the test substance of meta-Diisopropanolbenzene belongs to low toxic chemical for Daphnia magna.

Description of key information

Under valid test conditions mentioned above, the 48h EC50is greater than 150 mg/L (nominal concentration). And 48h NOEC value (no observed effect concentration) is greater than 150 mg/L (nominal concentration).

Key value for chemical safety assessment

Additional information