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Toxicity to soil macroorganisms except arthropods

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Reference
Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: - Not enough details on test organism. - No standard method for preparation of the test substrate and not enough details given, no information on mixing after addition of water.
Qualifier:
according to
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
Deviations:
yes
Remarks:
see Principles of method if other than guideline
Principles of method if other than guideline:
- Not enough details on test organism. - No standard method for preparation of the test substrate and not enough details given, no information on mixing after addition of water.
GLP compliance:
yes
Remarks:
GLP issued by State Environmental Protection Administration (SEPA) of the People’s Republic of China
Analytical monitoring:
not required
Vehicle:
no
Details on preparation and application of test substrate:
The test substance (meta-Diisopropanolbenzene) was directly added into the soil. A total amount of 10 g mixture of the test substance and dry soil was prepared firstly and then mixed with 740 g dry weight of soil for each test container.
The medium was thoroughly mixed and deionised water was added finally to give an overall moisture content of about 35 per cent of the dry weight. Moisture content was determined by drying a small sample at 105°C and re-weighing. The complete mixture was moist but not so wet that water appears when the artificial soil is compressed.
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
Eisenia foetida was adopted as the test species. The worms were obtained from an earthworm nursery of Nanjing city in China.
Before the test, the worms were acclimated in no polluted soil for 25 days. Worms for this study were adult (two months old with clitellum) with an individual weight of 320 mg. Batch No.: E20111115.
The worms were then pre-cultured in test soils for another 24h. The worms were washed quickly with distilled water and surplus water absorbed on filter paper before use.
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
yes
Total exposure duration:
14 d
Test temperature:
20 to 22 °C
pH:
6.0 to 6.2
Moisture:
32 to 36%
Details on test conditions:
TEST SYSTEM
- Test container (material, size): Glass spotless beakers, 1L
- Amount of soil or substrate: 750 g dry weight
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
Artificial soil test substrate was prepared as follows:
a) 10 per cent sphagnum peat (pH 6.0, no visible plant remains, finely ground, dried);
b) 20 per cent kaolin clay (kaolinite content preferably above 30 per cent);
c) 70 per cent industrial sand (fine sand should be dominant with 60 per cent of the particles between 50 and 200 microns);
d) pH is adjusted to 6.0 by addition of calcium carbonate.
The dry constituents were blended in the correct proportions and mixed thoroughly with a large-scale laboratory mixer.

OTHER TEST CONDITIONS
- Photoperiod: continuous light
- Light intensity: 400 lux to 800 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality

- Range finding study
- Test concentrations: 10, 100, 1000 mg/kg dw
- Results used to determine the conditions for the definitive study: no animals died during the range finding study.
Nominal and measured concentrations:
nominal: 1000 mg/kg dry weight
Reference substance (positive control):
yes
Remarks:
2-Chloracetamide
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
At the beginning of the administration, test worms soon disappeared into the test soil medium. During the test, all earthworms remained in the soil.
Results with reference substance (positive control):
14d-LC50 = 25.1 mg/kg soil dw
Reported statistics and error estimates:
In this test, no statistical method was adopted due to the low toxicity for the test substance to the test earthworm.
Validity criteria fulfilled:
yes
Conclusions:
The study was performed under Chinese GLP. The study can be used for risk assessment and classification and labelling, with some restrictions.
Executive summary:

The study was conducted to determine the acute toxicity of test substances (meta-Diisopropanolbenzene) to earthworm according to the guidelines of SEPAHJ/T 153 -2004, “the guidelines for the testing of chemicals”; and with the reference toProcedure 207 of the ‘Guidelines for Testing of Chemicals’ of the OECD: "Earthworm, Acute Toxicity Tests” (1984) etc.

During this test, the artificial soils was used with the moisture content of the test medium controlled in range of 32% to 36% with pH from 6.0 to 6.2. The test conditions were maintained as follows: temperature of 20oC to 22oC, humidity of 80% to 85% and continuous light with intensity of 400 lux to 800 lux.

Based on the result of range-finding test and the Limit Test, the14d LC50 of the test substance in the artificial soil was determined to be greater than 1000 mg/kg dry weight soil. The No-Observed-Effect Concentration (14d-NOEC) was greater than 1000 mg/kg dry weight soil. According to the toxicity catalogue of new chemicals, the test substance (meta-Diisopropanolbenzene) is low toxic to earthworms (Eisenia foetida).

Description of key information

Based on the result of range-finding test and the Limit Test,the14d LC50of the test substance in the artificial soil was determined to be greater than 1000 mg/kg dry weight soil. The No-Observed-Effect Concentration (14d-NOEC) was greater than 1000 mg/kg dry weight soil.

Key value for chemical safety assessment

Additional information