L-Aspartic acid, N,N'-1,2-ethanediylbis-, sodium salt (1:3)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From June 29, to July 09, 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0, 100, 320 and 1000 mg/L
- Sampling method: samples were taken at t = 0 h (newly prepared solutions) and t = 24 h (spent solutions after 24 h). Samples of ca. 100 mL were taken in polyethylene bottles and preserved by the addition of formol (1% final active concentration).
- Sample storage conditions before analysis: samples were stored in a refrigerator.

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Source: commercial hatchery Romberg's Aquariumhuis (Hugo de Grootplein 25, Delft)
- Weight at study initiation (mean and range, SD): 0.12 ± 0.02 g
- Length at study initiation (mean and range, SD): 2.5 ± 0.2 cm
- Feeding during test: no

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

None

Hardness:

224 mg/L (expressed as CaCO3)

Test temperature:

24.7-25.5oC (see Table 1 for details)

pH:

7.0-7.9 (see Table 2 for details)

Dissolved oxygen:

The lowest value measured was 7.0 mg/L (see Table 3 for details)

Salinity:

No data

Nominal and measured concentrations:

100, 180, 320, 560 and 1000 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: two L all-glass beakers, each containing 1.5 L of test solution or control medium.
- Aeration: Slightly aerated
- Renewal rate of test solution: replaced daily
- No. of organisms per vessel: ten
- No. of vessels per concentration: one
- No. of vessels per control: one


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: the dilution water used was DSWL, and prepared by addition of several salts to groundwater from a locality near Linschoten (The Netherlands).
- Total organic carbon: 1.9 mg/L
- Composition: The following components were measured by chemical analysis and found to be:
Na+ 1.33 mmol/L
K+ 0.20 mmol/L
Ca+2 1.42 mmol/L
Mg+2 0.82 mmol/L
Cl- 2.81 mmol/L
SO4-2 0.55 mmol/L


OTHER TEST CONDITIONS
- Photoperiod: 16 h light-8 h dark with transition periods of ca. 30 minutes


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, condition (swimming behaviour, colour, respiratory function or any other visually observable morphological or behavioural criterion) of the survivors were compared with that of the control animals.


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study
- Test concentrations: no data
- Results used to determine the conditions for the definitive study: no data

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: No deaths seen at highest concentration

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

ca. 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

ca. 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

ca. 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

morphology

Details on results:

- Behavioural abnormalities: no data
- Other biological observations: swimming behaviour, colour, respiratory function or any other visually observable morphological or behavioural criterion were comparable to that of the control animals.
- Mortality of control: none
- Other adverse effects control: no data
- Abnormal responses: no data

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

Not applicable

Sublethal observations / clinical signs:

Table 4: Number of fish and their condition in the control medium and in the test substance solutions during the test

Time (h)	Nominal concentration of test substance (mg/L)
	0	100	180	320	560	1000
0	10^a)	10^a)	10^a)	10^a)	10^a)	10^a)
24	10^a)	10^b)	10^b)	10^b)	10^b)	10^b)
48	10^a)	10^b)	10^b)	10^b)	10^b)	10^b)
72	10^a)	10^b)	10^b)	10^b)	10^b)	10^b)
96	10^a)	10^b)	10^b)	10^b)	10^b)	10^b)

where,

a) Condition of all fish, visually assessed, normal (= good)

b) Condition of all fish, visually assessed, equal to that of the control fish

Validity criteria fulfilled:

yes

Conclusions:

In a GLP study conducted according to OECD Guideline 203, trisodium EDDS was not acutely toxic to the freshwater fish, Brachydanio rerio, when tested up to a nominal concentration of 1000 mg/L, therefore the 96-h NOEC value for survival and condition was 1000 mg/L.

Executive summary:

A GLP study conducted according to OECD Guideline 203 was performed to determine the acute toxicity of trisodium EDDS to Brachydanio rerio (10 fish/concentration). The test was carried out under semi-static conditions with daily replacement of the test solutions. Fish were exposed to trisodium EDDS in freshwater at nominal concentrations of 0, 100, 180, 320, 560 and 1000 mg/L and observed for survival, condition (swimming behaviour, colour, respiratory function or any other visually observable morphological or behavioural criterion) of the survivors after 24, 48, 72 and 96 h of exposure.

After 96 h, all Zebrafish were alive and their condition was comparable to that of the control animals. All test solutions were completely clear (visually assessed) throughout the test.

 

In conclusion, the 96-h NOEC for survival and condition of this freshwater fish was 1000 mg/L (the highest tested nominal concentration), and the 96-h LC50 would therefore be >1000 mg/L.

Description of key information

In a GLP study conducted according to OECD Guideline 203, trisodium EDDS was not acutely toxic to the freshwater fish, Brachydanio rerio, when tested up to a nominal concentration of 1000 mg/L, therefore the 96-h NOEC value for survival and condition was 1000 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 1 000 mg/L

Additional information

A GLP study conducted according to OECD Guideline 203 was performed to determine the acute toxicity of trisodium EDDS to Brachydanio rerio (10 fish/concentration). The test was carried out under semi-static conditions with daily replacement of the test solutions. Fish were exposed to trisodium EDDS in freshwater at nominal concentrations of 0, 100, 180, 320, 560 and 1000 mg/L and observed for survival, condition (swimming behaviour, colour, respiratory function or any other visually observable morphological or behavioural criterion) of the survivors after 24, 48, 72 and 96 h of exposure. After 96 h, all animals were alive and their condition was comparable to that of the control animals. All test solutions were completely clear (visually assessed) throughout the test. In conclusion, the 96-h NOEC for survival and condition of freshwater fish was 1000 mg/L (the highest tested nominal concentration), and the 96-h LC50 would be >1000 mg/L (Hooftman and van Drongelen-Sevenhuijsen, 1993).

No data in marine fish currently available.