Reaction mass of N,N,N',N'-tetrabutylmethylenediamine and dibutylamine

Endpoint summary

Description of key information

Four valid acute experimental studies based on international guidelines are available to assess the aquatic toxicity of the registered substance.

Among all data available, toxic effects were observed on all aquatic trophic levels. The most sensitive species is the tested algae Pseudokirchneriella subcapitata, with a 72h-ErC50 and 72h-ErC10 of 13.94 and 4.19 mg/L, respectively. For the aquatic invertebrate Daphnia magna, the 48h-EC50 value was measured at 72.28 mg/L. For the fish Danio rerio, the LC50 was 57.11 mg/L.  In addition, the effect of the on the respiration of activated sewage sludge gave a 3h-EC50 and a 3h-EC10 value of 454.2 and 184.7 mg/L, respectively.

Supporting studies are also available for each endpoint. These studies were conducted in 2018 but were found to be unreliable due to analytical method shortcomings. For the studies conducted in 2018 it was not possible to measure N,N,N',N'-tetrabutylmethylenediamine (constituent 1), therefore, dibutylamine (constituent 2) was used as an indicator analyte of total concentration and stability of the whole substance. As such only effective loading rates could be derived and the stability of constituent 2 could not be confirmed.  However, new experimental data at each trophic level has shown that both constituents were stable during the course of the studies. The new studies are key studies, with effective concentrations derived and full analytical verification of both constituents of the multi-constituent susbtance.