Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 948-040-6
CAS number: -
Table 1 Mortality Data
Mean achieved atmosphere concentration
1 male killed in extremis due to evert signs of clinical toxicity
OECD 436 (2018) - In an acute inhalation toxicity study, groups of young
adult RccHan:WIST strain rats (6 male and 6 female) were exposed by
inhalation route to Reaction Mass of N,N,N',N-tetrabutylmethylenediamine
and dibutylamine for 4 hours to nose only at mean measured
concentrations of 1.02 and 5.67 mg/L. Animals then were observed
for 14 days after which time they were sacrificed and underwent necropsy.
In the 5.67 mg/L group, all 6 rats were found dead within 102-170
minutes exposure. There was no mortality observed in the 1.02 mg/L group.
5.67 mg/L group: During exposure all animals exhibited decreased
respiratory rate. One animal died after 102 minutes of exposure, two
animals died after 120 minutes of exposure. Labored respiration and
gasping respiration were also exhibited by two of the surviving animals
after 120 minutes of exposure. One animal died after 160 minutes of
exposure and a further animal died after 170 minutes of
exposure. Ataxia, hunched posture and areas of red/brown staining around
the snout were also exhibited by the surviving animal after 180 minutes
of exposure. This animal was humanely killed due to the occurrence of
clinical signs of toxicity that were considered likely to exceed the
severity limit set forth in the UK Home Office Project License after 230
minutes of exposure.
1.02 mg/L group: During exposure, on removal from the chamber and one
hour post-exposure, all animals exhibited decreased respiratory rate. On
removal from the chamber all animals exhibited ataxia, which was severe
in one animal, and there were occasional instances of gasping
respiration, labored respiration, noisy respiration, areas of red/brown
staining around the snout with isolated instances of clonic convulsions,
lethargy and areas of red/brown staining around the eyes. Improvement in
the condition of the animals was noted one hour post-exposure.
One day after exposure, all animals exhibited hunched posture and one
female also exhibited noisy respiration, pilo-erection and areas of
red/brown staining around the eyes.
Animals recovered to appear normal from Days 2 to 5 post-exposure. One
male that recovered to appear normal on Day 2 post-exposure subsequently
exhibited sneezing on Day 3 post‑exposure. Sneezing was also exhibited
by one female from Days 2 to 4 post-exposure.
5.67 mg/L group: None of the animals survived until the end of the day
of exposure and as such no significant body weight data was collected.
1.02 mg/L group: With the exception of one female, all animals showed
body weight loss on Day 1 post‑exposure. Animals showed expected gains
in body weight during the remainder of the recovery period with the
exception of two males that showed body weight loss or no gain in body
weight from Days 1 to 3 post-exposure, one female that showed no gain in
body weight from Days 3 to 7 post-exposure and one female that showed no
gain in body weight from Days 7 to 14 post-exposure.
5.67 mg/L group: The following macroscopic abnormalities were detected
at necropsy of animals that died or were humanely killed during exposure:
Lungs – hemorrhagic, pale and/or abnormally red.
1.02 mg/L group: No macroscopic abnormalities were detected at necropsy
of one animal that survived until the end of the recovery period.
The following macroscopic abnormalities were detected at necropsy of the
remaining animals that survived until the end of the recovery period:
Lungs – pale, dark patches, abnormally red, abnormally dark.
Due to the observations noted during the study and at necropsy it is
considered that the deaths noted during the study may have been mainly
attributable to local toxicity.
None of the animals died in a group of six rats exposed to a mean
achieved atmosphere concentration of 1.02 mg/L. However, all animals
died in a group of six rats exposed to a mean achieved atmosphere
concentration of 5.67 mg/L. It was therefore considered that the acute
inhalation median lethal concentration (4 hour LC50) of the test item,
in the RccHanTM : WIST strain rat, should be considered to be > 1.0 –
5.0 mg/L (Globally Harmonized Classification System – Category 4). This
equates to an LD50 based on the cut-off value of 2.5 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again