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EC number: 801-282-5 | CAS number: 1034343-98-0
Endpoint summary
Administrative data
Link to relevant study record(s)
Description of key information
Key value for chemical safety assessment
Additional information
In an available publication, dermal absorption of few layer graphene (FLG) was investigated in a skin vitro test system. The study was conducted according to national standard methods with acceptable restrictions.
In the study, histological analysis were carried out on hematoxylin/eosin stained RhE specimens after single exposure of FLG to study dermal absorption.
After material exposure, RhE specimens were collected, fixed and hematoxylin/eosin stained and analyzed under the Axio Scope A1, Axiocam 503, using the Zen2 software to observe morphological changes of the skin model and presence of FLG above and within the epidermis.
The results demonstrated the presence of aggregated/agglomerated FLG above the epidermis surface, showing small flat aggregates/ agglomerates for FLG. FLG depots, far smaller than the aggregates/agglomerates observed above the epidermis surface, were observed only within the RhE stratum corneum (composed of completely keratinized keratinocytes). This result suggests that highly dispersed not-aggregated/ agglomerated materials could penetrate into the stratum corneum but could be not able to pass through the stratum granulosum down to the stratum spinosum and stratum basale.
As FLG is not able to deeply penetrate into the inner layers of the epidermis, FLG is unable to directly interact with proliferating keratinocytes of the stratum basale.
In conclusion, histological analysis demonstrated the presence of small agglomerates of FLG above the epidermis. Small depots within the epidermis were observed but limited to the stratum corneum (these depots were fare smaller that the aggregates observable above the epidermis).
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