Sulphophthalic acid derivatives reaction products of fatty alcohol C12-C14 and sodium hydroxide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From May 30 to June 18, 1995

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992

GLP compliance:

not specified

Analytical monitoring:

no

Details on test solutions:

Test item was directly dispersed in water.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout.
- Source: Donnington Fish Farm, Upper Swell, Gloucester U.K.
- Length at study initiation: 4.9 ± 0.2 cm
- Weight at study initiation: 1.49 ± 0.20 g

ACCLIMATION
- Health during acclimation: no deaths during the 7 days prior to the study start.

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

14 °C

Nominal and measured concentrations:

Nominal 10 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 litres
- Aeration: yes.
- Renewal rate of test solution: daily.
- No. of vessels per concentration: 2 replicates.
- No. of vessels per control : 1 replicate.
- Biomass loading rate: 0.75 g bw/litre

TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: dechlorinated tap water (by passage through an activated carbon filter).
- Hardness: approximately 100 mg/l as CaCO3.

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 1.0, 10 and 100 mg/l
- No. of organisms per concentratyion: 3 fish.
- Results used to determine the conditions for the definitive study: initially a limit test was carried out at 100 mg/l. During the experiment progressive mortalities occurred due to the semi-static conditions employed as opposed to the static test conditions under which the range-finding test was carried out. On repeating the range-finding under semi-static conditions adverse reactions to exposure (i.e. 3/3 fish swimming at the bottom of the test vessel) were observed at the test concentration of 100 mg/l. Therefore, the definitive study was repeated at 10 mg/l to determine a true no effect concentration.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

10 - 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

DEFINITIVE TEST

Concentration (mg/l)	Cumulative mortality (initial population: 3)
Control	24 hrs	48 hrs	72 hrs	96 hrs
10	0	0	0	0
10	0	0	0	0

DOSE RANGE FINDING

Concentration (mg/l)	Cumulative mortality (initial population: 3)
Control	24 hrs	48 hrs	72 hrs	96 hrs
1	0	0	0	0
10	0	0	0	0
100	0	0	0	0*

*initially a limit test was carried out at 100 mg/l. During the experiment, progressive mortalities occurred due to the semi-static conditions employed as opposed to the static test conditions under which the range-finding test was carried out. On repeating the range-finding under semi-static conditions adverse reactions to exposure (i.e. 3/3 fish swimming at the bottom of the test vessel) were observed at the test concentration of 100 mg/l. Therefore, the definitive study was repeated at 10 mg/l to determine a true no effect concentration.

Conclusions:

LC50 (96h): 10 - 100 mg/l (nominal)

Executive summary:

The acute lethal toxicity of the test substance to rainbow trout was investigated under semi-static exposure conditions over a period of 96 hours, following the OECD guideline 203. Initially a limit test was carried out at 100 mg/l. During the experiment, progressive mortalities occurred due to the semi-static conditions employed as opposed to the static test conditions under which the range-finding test was carried out. On repeating the range-finding under semi-static conditions, testing the concentrations of 1, 10 and 100 mg/l, adverse reactions to exposure (i.e. 3/3 fish swimming at the bottom of the test vessel) were observed at the highest tested concentration. Therefore, the definitive study was repeated at 10 mg/l to determine a true no effect concentration.

No mortality was also observed in the blank control and in the test vessels.

The LC50 was indicated to be between 10 and 100 mg/l.

Conclusion

LC50 (96h): 10 - 100 mg/l (nominal)

Description of key information

Harmful for short-term exposure to fish; LC50 (96h) between 10 - 100 mg/l (nominal)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

10 mg/L

Additional information

The acute lethal toxicity of the test substance to rainbow trout was investigated under semi-static exposure conditions over a period of 96 hours, following the OECD guideline 203. Initially a limit test was carried out at 100 mg/l. During the experiment, progressive mortalities occurred due to the semi-static conditions employed as opposed to the static test conditions under which the range-finding test was carried out. On repeating the range-finding under semi-static conditions, testing the concentrations of 1, 10 and 100 mg/l, adverse reactions to exposure (i.e. 3/3 fish swimming at the bottom of the test vessel) were observed at the highest tested concentration. Therefore, the definitive study was repeated at 10 mg/l to determine a true no effect concentration. No mortality was also observed in the blank control and in the test vessels.

The LC50 was indicated to be between 10 and 100 mg/l.