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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from peer reviwed journal

Data source

Reference
Reference Type:
review article or handbook
Title:
AMES test for p-Anisaldehyde
Author:
National Institute of Technology and Evaluation.
Year:
2017
Bibliographic source:
Japan chemicals collaborative knowledge database (J-check), 2017

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: As mention below
Principles of method if other than guideline:
To evaluate the mutagenic potential of p-Anisaldehyde in Salmonella typhimurium TA100, TA1535, TA98, TA1537 and Escherichia
coli WP2 uvrA.
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid
Details on test material:
- Name of test material (as cited in study report): Anisic aldehyde/ p-Anisaldehyde
- Molecular formula: C8H8O2
- Molecular weight: 136.1492 g/mol
- Substance type: organic
- Physical state: liquid
-Smiles: COc1ccc(C=O)cc1
-InChI: 1S/C8H8O2/c1-10-8-4-2-7(6-9)3-5-8/h2-6H,1H3
Specific details on test material used for the study:
Details on test material
- Name of test material (as cited in study report): 4-Methoxybenzaldehyde
- Molecular formula: C8H8O2
- Molecular weight: 136.1492g/mol
- Substance type: organic

Method

Target gene:
Histidine
Species / strain
Species / strain / cell type:
other: Salmonella typhimurium TA100, TA1535, TA98, TA1537 and Escherichia coli WP2 uvrA.
Details on mammalian cell type (if applicable):
Not applicable.
Additional strain / cell type characteristics:
not specified
Cytokinesis block (if used):
not specified
Metabolic activation:
with and without
Metabolic activation system:
S9from Rat liver, induced with phenobarbital and 5,6-benzoflavone
Test concentrations with justification for top dose:
-S9 mix; 39.1, 78.1, 156, 313, 625, 1250 µg/plate(TA100, TA1535,
TA1537)
-S9 mix; 78.1, 156, 313, 625, 1250, 2500, 5000 µg/plate(WP2 uvrA)
-S9 mix; 156, 313, 625, 1250, 2500, 5000 g/µplate(TA98)
+S9 mix; 156, 313, 625, 1250, 2500, 5000 µg/plate(TA100, TA1535,
TA98, TA1537)
+S9 mix; 39.1, 78.1, 156, 313, 625, 1250, 2500, 5000 µg/plate(WP2
uvrA)
Vehicle / solvent:
Vehicle
- Vehicle(s)/solvent(s) used: DMSO
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
yes
Remarks:
DMSO
True negative controls:
not specified
Positive controls:
yes
Positive control substance:
other: -S9 mix; 2-(2-Furyl)-3-(5-nitro-2-furyl)acrylamide (TA100, TA98 and WP2 uvrA), Sodium azide (TA1535) and 9-Aminoacridine hydrochloride (TA1537) +S9 mix; 2-Aminoanthracene (all strains)
Details on test system and experimental conditions:
Details on test system and conditions
METHOD OF APPLICATION: Pre-incubation method
NUMBER OF REPLICATIONS: 2
- Other: 3 plates per test were observed.
Rationale for test conditions:
Not specified.
Evaluation criteria:
The test was considered to be positive, if there is increase in revertant colonies.
Statistics:
Not specified

Results and discussion

Test results
Species / strain:
other: Salmonella typhimurium TA100, TA1535, TA98, TA1537 and Escherichia coli WP2 uvrA.
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
valid
Untreated negative controls validity:
not specified
Positive controls validity:
valid
Remarks on result:
other: No mutagenic effect were observed.

Applicant's summary and conclusion

Conclusions:
4-Methoxybenzaldehyde(123-11-5) was evaluated for its mutagenic potential in Salmonella typhimurium TA100, TA1535, TA98, TA1537 and Escherichia coli WP2 uvrA. The test result was considered to be negative in the presence and absence of metabolic activation.
Executive summary:

Genetic toxicity in vitro study for p-Anisaldehyde was assessed for its possible mutagenic potential. For this purpose AMES test was performed according to Guidelines for Screening Mutagenicity Testing of Chemicals(Chemical Substances Control Law of Japan) and OECD Test Guideline 471 .The test material was exposed to Salmonella typhimurium TA100, TA1535, TA98, TA1537 and Escherichia coli WP2 uvr A at different concentration in the presence and absence of metabolic activation. No mutagenic effects were observed. Hence p-Anisaldehyde was considered to be non mutagenic in Salmonella typhimurium TA100, TA1535, TA98, TA1537 and Escherichia coli WP2 uvrA by AMES test .Hencethe test substance cannot be classified as gene mutant in vitro.