Registration Dossier
Registration Dossier
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EC number: 264-150-0 | CAS number: 63449-39-8
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1981
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- Ames (1975)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Paraffin waxes and Hydrocarbon waxes, chloro
- EC Number:
- 264-150-0
- EC Name:
- Paraffin waxes and Hydrocarbon waxes, chloro
- Cas Number:
- 63449-39-8
- Molecular formula:
- C18H33Cl5 C18H30Cl8 C20H36Cl6 C20H33Cl9 C25H45Cl7 C25H42Cl10 C25H29C23 C30H53Cl9 C30H49Cl13 C30H35Cl27
- IUPAC Name:
- Paraffin waxes and Hydrocarbon waxes (C18 and longer), chloro
- Details on test material:
- C22-26; 42% Cl (liquid). Cereclor 42.
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Arochlor-induced rat liver postmitochondrial supernatant (S-9 mix).
- Test concentrations with justification for top dose:
- 0.25 - 2500 µg/ml
Controls
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Positive control substance:
- other: 1,3-propanesultone for TA1535 and TA100; 2-acetylaminofluorene was for TA1538 and TA98.
- Evaluation criteria:
- The criteria for a positive response was taken to be a two-fold or greater increase in the mean number of revertant colonies appearing in the text plates over and above the background spontaneous reversion rate observed with the solvent, DMSO, together with evidence of a dose-response relationship.
Results and discussion
Test results
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Remarks on result:
- other: strain/cell type: S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and without activation
Negative with and without activation. - Executive summary:
Negative with and without activation.
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