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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2003
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Version / remarks:
and Directive CEE - Method C3
GLP compliance:
yes (incl. QA statement)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Limit test:
yes
Total exposure duration:
72 h
Remarks on exposure duration:
observations of cell growth at 24, 48 and 72h

Test conditions

Hardness:
LC Reconstituted water; hardness of 34 +/- 17 mg CaCO3/L.
Test temperature:
22.7 to 23.2°C
pH:
7.34 to 8.06
Nominal and measured concentrations:
See table below in the window: Any other information on the method incl. figures and tables.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
other: ErC50 (algae specific grow th rate)
Effect conc.:
> 37.1 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks:
Specific growth rate: natural log of the cell number over specific intervals (0-24 h, 0-48 h, 0-72 h)
Duration:
48 h
Dose descriptor:
other: ErC50 (algae specific groth rate)
Effect conc.:
> 35.7 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks:
Specific growth rate: natural log of the cell number over specific intervals (0-24 h, 0-48 h, 0-72 h)
Duration:
72 h
Dose descriptor:
other: ErC50 (algae specific growth rate)
Effect conc.:
> 34.2 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks:
Specific growth rate: natural log of the cell number over specific intervals (0-24 h, 0-48 h, 0-72 h)
Duration:
72 h
Dose descriptor:
other: EbC50
Effect conc.:
> 34.2 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks:
(cell.ml-1.h: increase in cell number/ml of solution x time (h)
Results with reference substance (positive control):
None.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
No Observed Effect Concentration (NOEC) at 72h: >= 34.2mg/L, based on specific growth rate and biomass data.
Not classified.